Simultaneous and Visual Detection of KPC and NDM Carbapenemase-Encoding Genes Using Asymmetric PCR and Multiplex Lateral Flow Strip

Table 1

Evaluation of KPC and NDM detection in 23 clinical samples of Enterobacteriaceae.

No.	Bacterial isolates	Carbapenemase inhibitor enhancement test (phenotypic tests)	LFT results		Sequencing
			KPC	NDM	KPC	NDM
1	E. coli	Metallo-ß-lactamase	N		N	NDM-1
2	E. asburiae	Noncarbapenemase producers	N	N	N	N
3	K. pneumoniae	Serine		N	KPC-2	N
4	K. pneumoniae	Serine		N	KPC-2	N
5	K. pneumoniae	Serine		N	KPC-2	N
6	K. pneumoniae	Serine		N	KPC-2	N
7	K. pneumoniae	Serine		N	KPC-2	N
8	K. pneumoniae	Serine		N	KPC-2	N
9	K. pneumoniae	Noncarbapenemase producers	N	N	N	N
10	K. pneumoniae	Serine		N	KPC-2	N
11	E. cloacae	Serine		N	KPC-2	N
12	E. coli	Metallo-ß-lactamase	N		N	NDM-1
13	K. oxytoca	Metallo-ß-lactamase	N		N	NDM-1
14	K. pneumoniae	Serine		N	KPC-2	N
15	K. pneumoniae	Serine		N	KPC-2	N
16	E. coli	Noncarbapenemase producers	N	N	N	N
17	K. pneumoniae	Serine		N	KPC-2	N
18	E. coli	Noncarbapenemase producers	N	N	N	N
19	K. pneumoniae	Metallo-ß-lactamase	N	P	N	NDM-1
20	K. pneumoniae	Serine		N	KPC-2	N
21	K. pneumoniae	Metallo-ß-lactamase	N		N	NDM-1
22	K. pneumoniae	Noncarbapenemase producers	N	N	N	N
23	K. pneumoniae	Serine		N	KPC-2	N