Self-Renewal Signalling in Presenescent Tetraploid IMR90 Cells
Figure 12
The antagonism between expression of NANOG and senescence regulators at the late presenescence and senescence phases: ((a)–(c)) late presenescence (MI = 0.1%): in the group of ten cells it is seen that nuclear staining of NANOG is found only in the cells with enlarged nuclei, where NANOG staining intensity is inverse to the staining for p21; ((d), (e)) senescence (MI = 0)—a rare NANOG-positive cell was encountered (arrowed), which has the weakest staining for p16 in its cytoplasm among five cells of the group. ((a), (d)) were imaged through the BRG filter; ((b), (e))—through blue, and (c)—through green filter. Scale bars = 10 μm.
