Self-Renewal Signalling in Presenescent Tetraploid IMR90 Cells
Figure 5
Examples of pre-senescent IMR90 fibroblasts grown on the coverslip, which differ from the surrounding cells by enlarged nuclei, cytoplasm volume, and the tendency of flattening. These cells express: (a) enhanced OCT4 (mostly in cytoplasm) and nuclear NANOG and (b) paranucleolarly localized NANOG, which is combined with enhanced expression of the senescence regulator, p16. Both, self-renewal and senescence regulators are undetectable or at the background level in the neighbouring fibroblasts possessing small nuclei. Scale bars = 10 μm. On (a) OCT4 was stained with the antibody for both A and B isoforms (Abcam polyclonal antibody, see Table 1).
