Self-Renewal Signalling in Presenescent Tetraploid IMR90 Cells
Figure 8
Mitoses of IMR90: (a) normal mitosis of a diploid cell in the growth stage, ((b)–(d)) mitoses in the presenescence stage. (b) Anaphase of the tetraploid cell, chromosome bridge (arrowhead) indicates to CIN. (c) Metaphase of the large tetraploid cell (~8C). (d) An attempt of the tetrapolar mitosis indicating to CIN. ((a),(b)) stoichiometric staining for DNA with Toluidine blue pH 4.0 after extraction of RNA. ((c),(d)) Similar staining after partial extraction of RNA. Scale bar = 10 μm.
