Morphofunctional and Biochemical Approaches for Studying Mitochondrial Changes during Myoblasts Differentiation

Table 3

Identification of mitochondrial protein differentially expressed during myogenesis.

No.	Protein	Score	NCBI nr	Peptides	MW	PI	Localization
1	Malate dehydrogenase, mitochondrial (MDH2)	162	DEMSMM	IFGVTTLDIVR,	35589	8.93	Mitochondrial matrix
				VDFPQDQLATLTGR,
				IQEAGTEVVK
2	Malate dehydrogenase precursor (MDH2)	345	DEMSMM	VAVLGASGGIGQPLSLLLK,	35589	8.93	Mitochondrial matrix
				IFGVTTLDIVRANTFVAELK,
				VDFPQDQLATLTGRIQEAGTEVVK,
				MIAEAIPELK
3	Aldehyde dehydrogenase 2, mitochondrial (Aldh2); putative  uncharacterized  protein	143	Q3TVM2_MOUSE	TIPIDGDFFSYTR,	56560	7.03	Mitochondrial matrix
				VAEQTPLTALYVANLIK,
				EAGFPPGVVNIVPGFGPTAGAAIASHEGVDK
4	Aldehyde dehydrogenase precursor, mitochondrial	411	I48966	TIPIDGDFFSYTR,	56502	7.53	Mitochondrial matrix
				LGPALATGNVVVMK,
				TFVQENVYDEFVER,
				TEQGPQVDETQFK,
				GYFIQPTVFGDVK,
				TIEEVVGR,
				YGLAAAVFTK
5	Voltage-dependent anion channel 1 (VDAC1)	161	VDAC1_MOUSE	LTFDSSFSPNTGK,	32331	8.55	Mitochondrial outer membrane
				VTQSNFAVGYK,
				LTLSALLDGK
6	Voltage-dependent anion channel 1 (VDAC1)	344	VDAC1_MOUSE	GYGFGLIK,	32331	8.55	Mitochondrial outer membrane
				WTEYGLTFTEK,
				LTFDSSFSPNTGK,
				VTQSNFAVGYK,
				VNNSSLIGLGYTQTLKPGIK,
				LTLSALLDGK
7	Pyruvate dehydrogenase (lipoamide) beta. (Pdhb protein)	288	Q99LW9_MOUSE	TYYMSAGLQPVPIVFR,	34814	5.63	Mitochondrial matrix
				DFLIPIGK,
				IMEGPAFNFLDAPAVR,
				VTGADVPMPYAK,
				VLEDNSVPQVK
8	Prohibitin	396	A39682	DLQNVNITLR,	29802	5.57	Mitochondrial intermembrane space
				ILFRPVASQLPR,
				IYTSIGEDYDER,
				VLPSITTEILK,
				FDAGELITQR,
				AAIISAEGDSK,
				AAELIANSLATAGDGLIELR,
				NITYLPAGQSVLLQLPQ
9	ATP synthase D chain, mitochondrial (ATP5H)	131	ATP5H_MOUSE	ANVAKPGLVDDFEK,	18607	5.52	Mitochondrial inner membrane
				YTALVDQEEKEDVK
10	Ubiquinol-cytochrome c reductase core protein 1	135	Q3THM1_MOUSE	TDLTDYLNR,	52806	5.89	Mitochondrial inner membrane
				IQEVDAQMLR
11	Fumarate hydratase precursor, mitochondrial (FH)	170	UFRT	AAAEVNQEYGLDPK,	54429	9.06	Mitochondrial
				AIEMLGGELGSK,
				VAALTGLPFVTAPNK
12	Superoxide dismutase precursor	72	DSRTN	GDVTTQVALQPALK	24659	8.96	Mitochondrial matrix
13	Aconitase 2, mitochondrial (ACO2)	340	Q3UDK9_MOUSE	DINQEVYNFLATAGAK,	85376	8.08	Mitochondrial matrix
				SQFTITPGSEQIR,
				NTIVTSYNR,
				FNPETDFLTGK,
				NAVTQEFGPVPDTAR,
				WVVIGDENYGEGSSR
14	Dihydrolipoamide dehydrogenase (DLDH)	139	Q99LD3_MOUSE	ADGSTQVIDTK,	54238	7.99	Mitochondrial matrix
				EANLAAAFGHPINF
15	ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit (ATP5B)	998	Q3TFD7_MOUSE	LVLEVAQHLGESTVR,	56207	5.25	Mitochondrial inner membrane
				TIAMDGTEGLVR,
				VLDSGAPIK,
				IPVGPETLGR,
				IMNVIGEPIDER,
				VVDLLAPYAK,
				IGLFGGAGVGK,
				TVLIMELINNVAK,
				EGNDLYHEMIESGVINLK,
				VALVYGQMNEPPGAR,
				VALTGLTVAEYFR,
				FTQAGSEVSALLGR,
				AIAELGIYPAVDPLDSTSR,
				IMDPNIVGNEHYDVAR,
				ILQDYK,
				FLSQPFQVAEVFTGHMGK
16	Protein disulfide isomerase A3 (Pdia3)	94	PDIA3_MOUSE	DASVVGFFR,	56472	5.88	Endoplasmic reticulum, also present in mitochondria (see discussion).
				GFPTIYFSPANK,
				ELNDFISYLQR