Proteomic Profiling of Mitochondrial Enzymes during Skeletal Muscle Aging

Table 2

Proteomic identification of mitochondrial proteins during skeletal muscle aging.

Proteomic study	Changes in mitochondrial marker proteins	Reference
Analysis of total extracts from aged human vastus lateralis muscle	General increase in aerobic markers, including mitochondrial enzymes such as ATP synthase, ubiquinol-cytochrome c reductase, and oxoglutarate dehydrogenase during muscle aging	Gelfi et al. [86]
Analysis of total extracts from rat gastrocnemius muscle	Increase in mitochondrial enzymes, such as succinate dehydrogenase, isocitrate dehydrogenase, ATP synthase, and malate dehydrogenase during muscle aging	Doran et al. [93]
Analysis of total extracts from rat gastrocnemius muscle	Differential effect on the abundance of mitochondrial isoforms of aconitase during muscle aging	O’Connell et al. [87]
Analysis of total extracts from aged rat gastrocnemius muscle	Moderate effect on cytochrome c oxidase and isocitrate dehydrogenase during muscle aging	Piec et al. [85]
Analysis of total extracts from rat gastrocnemius muscle	Increase in many enzymes involved in oxidative metabolism, such as ATP synthase, isocitrate dehydrogenase, ubiquinol-cytochrome c reductase, and pyruvate dehydrogenase during muscle aging	Capitanio et al. [94]
Subproteomic study of the effect of aging and caloric restriction on rat muscle mitochondria	Increased levels of isocitrate dehydrogenase and malate dehydrogenase in aged muscle mitochondria. Caloric restriction appears to have only a marginal effect on the mitochondrial proteome	Chang et al. [90]
Subproteomic analysis of mitochondria-enriched fraction from aged rat gastrocnemius muscle	Increased levels of mitochondrial creatine kinase, NADH dehydrogenase, ATP synthase, succinate dehydrogenase, and ubiquinol cytochrome c reductase during muscle aging	O’Connell and Ohlendieck [97]
Analysis of total extracts and mitochondria-enriched fraction from aged ratgastrocnemius muscle	Differential effect on mitochondrial enzymes, such as pyruvate dehyrdogenase, cytochrome c oxidase, isocitrate dehydrogenase, and ATP synthase during muscle aging	Lombardi et al. [96]
Subproteomic analysis of mitochondria-enriched fraction from aged mouse hind limb muscles	Differential effects on the abundance and carbonylation of various mitochondrial enzymes, including NADH dehydrogenase, cytochrome c oxidase, and ATP synthase during muscle aging	Alves et al. [98]
Analysis of detergent phase-extracted protein complement from aged rat gastrocnemius muscle	Increase in mitochondrial marker enzymes, such as ATP synthase and isocitrate dehydrogenase during muscle aging	Donoghue et al. [99]
Proteomic analysis of nitration in aged rat skeletal muscle	Increased nitration levels in succinate dehydrogenase	Kanski et al. [84]
Phosphoproteomic analysis of total extracts from aged rat gastrocnemius muscle	Decreased phosphorylation levels in cytochrome c oxidase and aconitase during muscle aging	Gannon et al. [92]
Proteomic analysis of carbonylation in aged rat skeletal muscle mitochondria	Altered carbonylation levels in numerous mitochondrial proteins, including ATP synthase, NADH dehydrogenase, pyruvate dehydrogenase, and isocitrate dehydrogenase during muscle aging	Feng et al. [91]