538979.fig.005a
(a)
538979.fig.005b
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Figure 5: Letrozole and reduce synaptic proteins. (a) Representative 3D reconstructions of dendritic segments from sister cultures that were treated with either control, (1 μM), and letrozole (1 μm) or + letrozole (1 μm) for 24 or 72 hours and immunostained for synaptophysin (white) and synaptopodin (red). Scale bar, 2 μm. (b) Quantification of the densities of synaptopodin-positive puncta following treatments. There is a significant decrease in synaptopodin puncta after 24 and 72 hours of , letrozole or + letrozole treatments compared to control conditions. After 72 hours, the number of synaptopodin puncta was significantly lower in letrozole and + letrozole-treated cultures compared to alone. When synaptopodin puncta densities were compared between 24- and 72-hour treated cultures there was a significant decrease only in letrozole and + letrozole-treated cultures after 72 hours. 24 hours: control, n = total dendritic segment lengths of 1041 μm from 12 cells in 4 cultures; , μm of dendrite from 8 cells in 4 cultures; letrozole, μm of dendrite from 10 cells in 4 cultures; + letrozole, μm of dendrite from 10 cells in 4 cultures. 72 hours: control, = total dendritic segment lengths of 559 μm from 10 cells in 3 cultures; , μm of dendrite from 9 cells in 4 cultures; letrozole, μm of dendrite from 9 cells in 4 cultures; + letrozole, μm of dendrite from 8 cells in 4 cultures. There is a significant decrease in synaptophysin puncta after 24 and 72 hours of , letrozole, or + letrozole treatments compared to control conditions. After 72 hours, the number of synaptophysin puncta was significantly lower in letrozole and + letrozole-treated cultures compared to alone When synaptophysin puncta densities were compared between 24- and 72-hour treated cultures there was a significant decrease in , letrozole, and + letrozole-treated cultures after 72 hours. 24 hours: control, n = total dendritic segment lengths of 1041 μm from 12 cells in 4 cultures; , μm of dendrite from 8 cells in 4 cultures; letrozole, μm of dendrite from 10 cells in 4 cultures; + letrozole, μm of dendrite from 10 cells in 4 cultures. 72 hours: control, n = total dendritic segment lengths of 559 μm from 10 cells in 3 cultures; , μm of dendrite from 9 cells in 4 cultures; letrozole, μm of dendrite from 9 cells in 4 cultures; + letrozole, μm of dendrite from 8 cells in 4 cultures.