Effect of Caloric Restriction on Hepatic Sinusoidal System and Stellate Cells in Mice
Figure 3
RT-PCR of profibrogenic proteins. Gene expressions of -Smooth Muscle Actin (-SMA, product size = 446 bp) and -1(I) collagen (product size = 174 bp). The house keeping gene, glyceraldehyde-3-phosphate dehydrogenase (GAPDH, product size = 346 bp) was used to establish equal loading. The calorically restricted (CR) mice () has decreased expression of -1(I) collagen as compared with mice fed ad libitum (Ctrl, ).