Research Article
Improved and Reproducible Flow Cytometry Methodology for Nuclei Isolation from Single Root Meristem
Table 1
Data of nuclear FCM analysis from root meristem according to methodology employed.
| Method-gentle hand-homogenization | Meristem source | Root tip length (mm) | Root tip/sample | CV (%) | Nuclei/sample | Species | References |
| Root | 0–2 | 100 (unclear) | 4.4 and 5.8 | | Pisum sativum | [21] |
| Method-glass rod |
| Root | 0–2 | 10 | 2.6 (only control) | — | Pisum sativum | [23] | Root | — | — | — | | Oryza sativa | [24] |
| Method-chopping |
| Radicle | 1 | 5–10 | — | 821–3852 | Solanum lycopersicum | [11] | Radicle | 1 | 20 | — | | Capsicum annuum | [12] | Radicle | — | 5 | — | — | Solanum lycopersicum | [13] | Radicle | — | 70–80 | — | ~ | Solanum lycopersicum | [14] | Radicle | 1 | 5 | — | | Solanum lycopersicum | [15] | Root | — | 10–30 | — | (unclear) | Arabidopsis thaliana | [26] | Root | — | — | — | — | Solanum tuberosum | [27] | Root | ~ 2 | 9 | — | | Allium cepa | [28] | Root | 2–3 | 20 | | | Secale cereale | [29] | Root | 2–3 | 20 | — | | Triticum aestivum | [29] | Root | 2–3 | 7 | | | Vicia faba | [29] | Root | 2 | 9 | — | | Allium cepa | [30] | Radicle | 1 | 3–5 | — | 3469, 4425, 7547, 18874 | various | [31] | Radicle | — | 15 | — | | Hordeum vulgare | [32] |
| Method-mechanical homogenizer |
| Root | 1–2 | — | — | — | Various | [1] | Root | 1 | 1 (and 1) | — | /mL | Pisum sativum | [5] | Root | 0.5–1 | 50 | — | ~ | Various | [19] | Root | 2 | — | — | — | Vicia faba | [33] |
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CV: coefficient of variation; —: data not shown.
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