Improved and Reproducible Flow Cytometry Methodology for Nuclei Isolation from Single Root Meristem

Table 1

Data of nuclear FCM analysis from root meristem according to methodology employed.

Method-gentle hand-homogenization
Meristem source	Root tip length (mm)	Root tip/sample	CV (%)	Nuclei/sample	Species	References
Root	0–2	100 (unclear)	4.4 and 5.8	5×10^6-1×10^7	Pisum sativum	[21]
Method-glass rod
Root	0–2	10	2.6 (only control)	—	Pisum sativum	[23]
Root	—	—	—	≥1×10^4	Oryza sativa	[24]
Method-chopping
Radicle	1	5–10	—	821–3852	Solanum lycopersicum	[11]
Radicle	1	≥20	—	≥1×10^4	Capsicum annuum	[12]
Radicle	—	5	—	—	Solanum lycopersicum	[13]
Radicle	—	70–80	—	~ 6×10^3	Solanum lycopersicum	[14]
Radicle	1	5	—	5×10^3-1×10^4	Solanum lycopersicum	[15]
Root	—	10–30	—	1×10^4 (unclear)	Arabidopsis thaliana	[26]
Root	—	—	—	—	Solanum tuberosum	[27]
Root	~ 2	9	—	8×10^3-1×10^4	Allium cepa	[28]
Root	2–3	20		8×10^3-1×10^4	Secale cereale	[29]
Root	2–3	20	—	8×10^3-1×10^4	Triticum aestivum	[29]
Root	2–3	7		8×10^3-1×10^4	Vicia faba	[29]
Root	∼2	9	—	8×10^3-1×10^4	Allium cepa	[30]
Radicle	1	3–5	—	3469, 4425, 7547, 18874	various	[31]
Radicle	—	15	—	1×10^4	Hordeum vulgare	[32]
Method-mechanical homogenizer
Root	1–2	—	—	—	Various	[1]
Root	1	1 (and >1)	—	1×10^5/mL	Pisum sativum	[5]
Root	0.5–1	50	—	~1.5×10^5	Various	[19]
Root	2	—	—	—	Vicia faba	[33]

CV: coefficient of variation; —: data not shown.