Fluorescence and Photochemical Investigations of Phytochrome in Higher Plants
Figure 18
Low-temperature (85 K) fluence-response curves for changes in phytochrome Pr concentration during its phototransformation into lumi-R (a) and back (b) at 85 K in etiolated (a) and preilluminated with saturating red light at 85 K (b) pea stems upon illumination with red and far-red light, respectively. The curves are obtained from the changes in Pr fluorescence (, ) as in (a) and as in (b). ( and are the Pr fluorescence intensity at photoequilibrium between Pr and lumi-R under saturating red ( nm) and far-red ( nm) light, respectively; is the fluorescence intensity of Pr at various times of illumination with red ( nm) or far-red ( nm) light. Parts (c) and (d) present deconvolution of the experimental curves in (a) and (b), respectively, in semilogarithmic coordinates. (From [89]).
