Leaves and Seeds as Materials for Flow Cytometric Estimation of the Genome Size of 11 Rosaceae Woody Species Containing DNA-Staining Inhibitors

Table 2

2C DNA content of leaves and seeds of trees and shrubs belonging to the Rosaceae family.

Species name	Internal standard*	Buffer**	2C DNA (pg, mean ± SD)
			Leaves	Seeds
Amelanchier stolonifera	2	B	2.59±0.02a^∗∗∗	2.55±0.01b
Cotoneaster dammeri	2	C	1.41±0.01^ns	1.41±0.02
Cotoneaster divaricatus	2	C	2.78±0.01a	2.73±0.02b
Cotoneaster hjelmqvistii	2	C	2.74±0.03a	2.70±0.01b
Cotoneaster horizontalis	2	C	2.77±0.03^ns	2.77±0.02
Cotoneaster veitchii	2	C	2.67±0.02a	2.63±0.02b
Crataegus coccinea	2	B	3.16±0.02^ns	3.17±0.02
Malus floribunda	1	B	1.45±0.01^ns	1.44±0.01
Prunus padus	1	B	1.15±0.02^ns	1.15±0.02
Pyrus elaeagrifolia	1	A	1.15±0.01^ns	1.15±0.02
Sorbus intermedia	2	B	2.82±0.01^ns	2.81±0.02

^∗1: Petunia hybrid; 2: Zea mays’; **A: Galbraith’s + 1% (w/v) PVP-10; B: Galbraith’s + 1.5% (w/v) PVP-10; C: Galbraith’s + 2% (w/v) PVP-10 + 15 mM 𝛽-mercapthoethanol; ***2C-values in leaves and seeds of the certain species (in lines) followed by different letters are significantly different at 𝑃=.05 (Student’s t-test); ns: no significant difference.