Leaves and Seeds as Materials for Flow Cytometric Estimation of the Genome Size of 11 Rosaceae Woody Species Containing DNA-Staining Inhibitors

Table 4

Quality of the histograms of nuclei isolated from leaves and seeds of trees and shrubs belonging to the Rosaceae family. CV: coefficient of variation for the 𝐺₀/𝐺₁ peak; DF: debris background factor.

Species name	CV of target species (%, mean ± SD)		CV of internal standard (%, mean ± SD)		DF (%, mean ± SD)
	Leaves	Seeds	Leaves	Seeds	Leaves	Seeds
Amelanchier stolonifera	6.54±0.52a^∗	4.43±0.43b	4.09±0.41a	4.63±0.31b	57.8±4.3a	22.2±3.6b
Cotoneaster dammeri	5.73±0.68a	3.83±0.39b	2.82±0.37^ns	3.12±0.30	78.6±8.7^ns	67.0±18.5
Cotoneaster divaricatus	4.52±0.21a	3.35±0.70b	3.23±0.51^ns	3.36±0.45	83.1±14.4^ns	78.0±4.9
Cotoneaster hjelmqvistii	4.58±0.54a	4.04±0.25b	3.53±0.41a	3.98±0.31b	56.2±10.3a	38.8±3.5b
Cotoneaster horizontalis	5.66±0.50a	3.95±0.40b	4.17±0.56a	3.64±0.33b	73.9±4.3a	40.6±2.8b
Cotoneaster veitchii	4.52±0.57a	3.40±0.76b	3.25±0.52^ns	3.51±0.58	75.6±13.2^ns	66.1±22.4
Crataegus coccinea	5.68±0.59a	4.06±0.35b	4.46±0.48a	3.42±0.52b	47.1±8.8a	31.6±8.5b
Malus floribunda	5.84±0.74a	4.45±0.25b	3.78±0.41^ns	3.79±0.41	45.2±6.3a	30.1±3.1b
Prunus padus	5.94±0.42^ns	5.95±0.45	3.60±0.39^ns	3.64±0.40	52.8±10.2^ns	53.9±7.8
Pyrus elaeagrifolia	5.86±0.71a	4.82±0.17b	3.81±0.36a	3.36±0.19b	44.2±6.9a	25.5±8.8b
Sorbus intermedia	5.78±0.42a	4.36±0.46b	3.92±0.30a	4.28±0.36b	53.4±9.26a	34.2±6.0b

∗Values for leaves and seeds of the certain species and for the certain parameter (in lines) followed by different letters are significantly different at 𝑃=.05 (Student’s t-test); ns: no significant difference.