- About this Journal ·
- Abstracting and Indexing ·
- Aims and Scope ·
- Article Processing Charges ·
- Author Guidelines ·
- Bibliographic Information ·
- Citations to this Journal ·
- Contact Information ·
- Editorial Board ·
- Editorial Workflow ·
- Free eTOC Alerts ·
- Publication Ethics ·
- Recently Accepted Articles ·
- Reviewers Acknowledgment ·
- Submit a Manuscript ·
- Subscription Information ·
- Table of Contents
Journal of Botany
Volume 2011 (2011), Article ID 570319, 9 pages
Evolution of Genome Size in Duckweeds (Lemnaceae)
1Rutgers, Department of Plant Biology and Pathology, The Waksman Institute of Microbiology, The State University of New Jersey, Piscataway, NJ 08854, USA
2Monsanto Company, 800 North Lindbergh Boulevard, Creve Coeur, MO 63167, USA
Received 4 February 2011; Revised 5 April 2011; Accepted 19 May 2011
Academic Editor: Johann Greilhuber
Copyright © 2011 Wenqin Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
To extensively estimate the DNA content and to provide a basic reference for duckweed genome sequence research, the nuclear DNA content for 115 different accessions of 23 duckweed species was measured by flow cytometry (FCM) stained with propidium iodide as DNA stain. The 1C-value of DNA content in duckweed family varied nearly thirteen-fold, ranging from 150 megabases (Mbp) in Spirodela polyrhiza to 1,881 Mbp in Wolffia arrhiza. There is a continuous increase of DNA content in Spirodela, Landoltia, Lemna, Wolffiella, and Wolffia that parallels a morphological reduction in size. There is a significant intraspecific variation in the genus Lemna. However, no such variation was found in other studied species with multiple accessions of genera Spirodela, Landoltia, Wolffiella, and Wolffia.
The Lemnaceae, commonly known as duckweeds, are the smallest, fastest-growing, and simplest of flowering plants. In this globally distributed aquatic monocot family (Figure 1(a)), there are 33 species representing five genera: Spirodela, Landoltia, Lemna, Wolffiella, and Wolffia. Among them, Spirodela is the most ancestral, while Wolffia is the most derived . The individual plants range in size from 1.5 cm long (Spirodela polyrhiza) to less than one millimeter (Wolffia globosa). Therefore, there is a successive reduction of morphological structures in parallel with evolutionary advancement within the family (Figure 1(b)). Duckweeds are not simply miniature versions of larger angiosperms; they represent a highly modified structural organization that resulted from the alteration, simplification, or loss of many morphological and anatomical features . The biomass doubling time of the fastest-growing duckweeds in optimal growth conditions is less than 30 hours, nearly twice as fast as other “fast” growing flowering plants and more than double that of conventional crops .
Before the days of Arabidopsis, duckweeds, and more specifically Lemna, were an important model system for plant biology . Since duckweeds are small, morphologically reduced (although with root and leaf-like structure), fast growing, easily cultivated under aseptic conditions (Figure 1(c)), transformable, crossable, and particularly suited to biochemical studies (direct contact with media), it is an ideal system for biological research . Much of what we know about photoperiodic flowering responses comes from fundamental research conducted on Lemna by the preeminent plant biologist Dr. William Hillman at the Brookhaven National Laboratories . Some of the current uses of Lemnaceae are a testimony to its scientific, commercial, and biomass utility: basic research and evolutionary model system , toxicity testing organism , biotech protein factories , wastewater remediation , high protein animal feed, carbon cycling , and biofuel potential candidates .
The advent of high-throughput sequencing technologies has enabled a new generation of model plant systems . In an effort to initiate duckweed genomic research, we endeavoured to identify species with small genomes that would be ideal for sequencing. First, we queried the Kew plant genome database (http://data.kew.org/cvalues/) and found that there were only 6 duckweed accessions that had been measured by the Feulgen method [13, 14]. DNA content of single species from each genus was determined and showed obvious difference. Due to it being laborious and time consuming, the popularity of Feulgen technique has waned. Feulgen has been largely replaced by flow cytometry (FCM) , a faster, easier, and more accurate method and the current preferred technique for genome size estimations and DNA ploidy analyses in plants .
In order to find the smallest duckweed genome for sequencing and also explore previous observations about genome complexity in duckweeds, we estimated the genome size of all of the five duckweed genera using FCM. These genome size measurements will form the foundation for future work in sequencing duckweed genome, and enabling duckweeds as a model and applied system.
2. Materials and Methods
2.1. Plant Materials
115 accessions of 23 duckweed species representing all 5 genera were measured in this study (Table S1 in Supplementary Materials available online at http://dx.doi.org/10.1155/2011/570319) (They provide details of sample collection and results of nuclear DNA content measurements for Lemnaceae.). Elias Landolt collected most of the duckweed accessions described in this work over the past 50 years (Landolt Duckweed Collection) . Accessions were either obtained directly from Elias Landolt, BIOLEX (NC, USA) or The University of Toronto Culture Collection of Algae and Cyanobacteria (UTCC). Currently, the Landolt Duckweed Collection has been moved to Rutgers University. Additional lines were collected from lakes and wastewater ponds by TPM and WW (NJ, USA). Plants were grown aseptically for 2 weeks with 1/2 full concentration of Schenk and Hildebrandt Basal Salt mixture (Sigma, USA) liquid culture medium under short day growth condition (8 h light and 16 h darkness with constant temperature 23°C). We bar-coded all the determined and undetermined species by identification of polymorphisms of chloroplast atpF-atpH noncoding spacer .
2.2. Isolation and Staining of Nuclei
To estimate nuclear DNA contents with flow cytometry (FCM), sample tissue nuclei were stained with propidium iodide (PI) . Briefly, 10 mg of fresh duckweed tissue and the same amount of the internal standard were chopped simultaneously with new razor blades and isolation buffer in a plastic Petri dish . Isolates were filtered through a 30-μm nylon mesh into an Eppendorf tube. The suspensions of nuclei were stained with 50 μg mL−1 PI mixed with 50 μg ml−1 RNase (R4875, Sigma). The samples were incubated on ice for a few minutes before estimation by FCM.
2.3. Analysis of Nuclear DNA Content by FCM
PI-stained nuclei were analyzed for DNA content with a Coulter Cytomics FC500 Flow Cytometer (Beckman Coulter, Inc., Miami, Florida, USA). In all experiments, the fluorescence of at least 3000 G1-phase nuclei was measured. DNA content of each target sample was calculated by comparing its mean nuclear fluorescence with that of an internal standard (Figure 2(a)). We utilized internal controls that closely match the duckweed genome sizes being measured to ensure accuracy. The internal standard is a Brachypodium distachyon line, (Bd21, 300 Mbp) , Arabidopsis thaliana Columbia., (At, 147 Mbp) , and Physcomitrella patens ssp patens, (Pp, 480 Mbp) . The numbers in bracket were generated by our flow cytometry equipment and our methods. Therefore, the validated genome sizes are not exactly the same but very close to cited references. Both duckweed and internal standards have very little secondary compounds, which will interfere with quantitative DNA staining. The absolute DNA content of a sample is calculated based on the values of the G1 peak means:
At least, three independent biological replicates for each sample were analyzed on different days to estimate the mean DNA content. The transformation factor from pg to Mbp is: 1 pg = 978 Mbp .
2.4. Statistical Analysis
Data on intraspecies variation of genome size were analysed by ANOVA: single factor test. To test whether genome size variation was correlated with geographic location or altitude of populations, the Spearman correlation coefficient (r) was used.
3.1. Intra- and Interspecies Variations of Genome Sizes
The genome sizes of 115 accessions from 23 species representing 5 genera were estimated by FCM (Table S1). The DNA content estimates varied nearly thirteen-fold, ranging from 150 Mbp in Spirodela polyrhiza to 1,881 Mbp in Wolffia arrhiza. We superimposed the estimated 1C-value on a phylogenetic tree for Lemnaceae based on combination of morphological, flavonoid, allozyme, and DNA sequence analysis  and found that there is a continuous increase of DNA content in order of Spirodela, Landoltia, Lemna, Wolffiella, and Wolffia, which correlates well with the morphological reduction within the family (Figures 3(a) and 3(b)).
In the genus Spirodela, we measured genome size for 34 accessions and found that the 1C DNA content only varies from 150 to 167 Mbp (Figure 3(a); Table S1). The analysis of variance (ANOVA: single factor test) revealed that there was not a significant difference in Spirodela polyrhiza genome sizes (). Similarly, the 1C DNA content for 19 accessions of Landoltia punctata from 372 to 397 Mbp did not show significant variation (Figure 3(a); Table S1). In the genus Wolffiella, the genome sizes range from 623 Mbp to 973 Mbp (Figure 3(a)), which is almost as 4–6 times large as Spirodela polyrhiza. Like Spirodela polyrhiza and Landoltia punctata, there are no obvious intraspecific genome size variations in Wolffiella hyalina and Wolffiella lingulata. In the genus Wolffia, we measured 11 species and found that they have the largest genome sizes on average among the duckweed family (Figure 3(a)). 5.3-fold difference was observed from Wolffia australiana (357 Mbp) to Wolffia arrhiza (1,881 Mbp).
In the genus Lemna, 7 species were investigated. There is a large amount of genome size variation in this genus. Lemna valdiviana has the smallest genome size (323 Mbp), while Lemna aequinoctialis has the biggest (760 Mbp). Surprisingly, intraspecific genome-size fluctuations are also impressive. For Lemna minor, 26 accessions have genome sizes ranging from 356 to 604 Mbp with up to 69.6% of the intraspecific DNA content variance. We confirmed the intraspecific difference of them by randomly choosing 2 Lemna minor with simultaneous measurement of both accessions (26.0% difference between 6591 Lm and 7436 Lm, Figure 2(b)). Statistical analyses revealed significant differences among the Lemna minor accessions (). As well, Lemna aequinoctialis (424–760 Mbp, 79.2%) (Figure 2(c)), Lemna trisulca (446–709 Mbp, 59.0%), and Lemna japonica (426–600 Mbp, 40.8%) all show intraspecific difference, indicating a drastically uneven evolution of intraspecific genome expansion in Lemna.
3.2. 1C-Value and Latitude, Longitude, and Altitude
To investigate whether there is a correlation between genome-size variations and the geographic distribution in the duckweed, we compared genome size estimates with the latitude, longitude, and altitude of recorded collection. However, genome size variation was not correlated with latitude by Pearson coefficient (-value: Spirodela = −0.05, Landoltia = 0.17, Lemna = −0.07, Wolffiella = −0.17, Wolffia = 0.34) (Figure 4(b)), nor with longitude (-value: Spirodela = 0.17, Landoltia = 0.04, Lemna = 0.26, and Wolffia = −0.41) (Figure 4(c)) except Wolffiella with a high -value −0.86 possibly due to limited accessions (). No correlation was found between C-values and altitude, either (-value: Spirodela = 0.13, Landoltia = −0.25, Lemna = −0.33, Wolffiella = −0.41, and Wolffia = 0.13) (Figure 4(d)). It is interesting we found that most of Spirodela, Landoltia, Wolffiella, and Wolffia were collected from a similar geographic range between 0° to 45° and preferred to localize above 600 m to 1200 m of altitude. In contrast, most of Lemna species were collected between 30° to 60° and preferred to distribute below 600 m. However, this most likely represents a sampling bias and could also explain the absence of a relationship between genome size and the environment in duckweed.
4.1. Genome Evolution in Duckweeds
In the phylogeny of Lemnaceae, there is a strong relationship observed between genome size evolution and morphological progression. We found that the ancestral genus Spirodela has the smallest genome size, while the most advanced genus Wolffia contains biggest genome size (Figure 3; Table S1), which correlates with the morphological reduction rather than organism complexity within the family. This result is consistent with Geber’s finding, which showed that there was a relationship between DNA content and degree of primitivity .
Genome doubling has been a pervasive force in plant evolution, which has occurred repeatedly . Even the smaller genome of Arabidopsis thaliana has been impacted by genome duplication . Cytological variation by counting the chromosomes was extensively investigated within duckweed. They concluded that polyploidy () is the main intrapopulational variation , which means polyploidization was very active and occurred in the duckweeds for multiple rounds in the past. After polyploidization, transposable element mobility, insertions, deletion, and epigenome restructuring contribute to the successful development of a new species and also genome size changes . Changes in genome structure could lead to differential gene loss, extensive changes in gene expression , and have immediate effects on the phenotype and fitness of an individual . It is likely polyploidy might drive the divergence during duckweed evolution.
4.2. Geographic Distribution and Genome Size Variation
It was suggested that variation in DNA content has adaptive significance and is correlated with the environmental traits of species . The environmental conditions of plants are to a large extent determined by latitude, longitude, and altitude. Previous studies have indicated a positive correlation between genome size and latitude (associated with the length of sun light with the growing season and the temperature) and also altitude (associated with the temperatures) among plant species. For example, the increase of DNA content corresponded with the increasing latitude found in the Pinaceae family  and with increasing altitude observed in Zea mays . Duckweeds are distributed broadly around the world (Figure 4(a)). Our result shows that there is no significant overall correlation of genome size with latitude, longitude, and altitude (Figure 4). The same result was found in Vicia faba , Sesleria albicans , and Asteraceae . A summary revealed that these relationships were not straightforward and not clear. Five studies (Picea sitchensis, Berberis, Poaceae, and Fabaceae, Tropical versus temperate grasses, 329 tropical versus 527 temperate plants) found positive, seven (Arachis duranensis, Festuca arundinacea, North American cultivars of Zea mays, 162 British plants, 23 Arctic plants, 22 North American Zea mays, and 11 North American Zea mays) found negative, and five (Allium cepa, Dactylis glomerata, and Helianthus) found nonsignificant correlations between genome size and latitude. Additionally, nine were positive, eight were negative, and six were not statistically significant between genome size and altitude . But the different environmental distribution of the Lemna genus (30° to 60° of latitude and below 600 m of altitude) with the other four duckweed genera (0° to 45° of latitude and 600 m to 1200 m of altitude) might explain the large intraspecific genome size variation.
4.3. Intraspecific Variation in Genome Size
Intraspecific genome consistency has been reported in Allium cepa , Glycine max , and Capsicum and Gossypium . We also found a similar result for Spirodela polyrhiza, Landoltia punctata, Wolffiella hyalina, Wolffiella lingulata, and Wolffia australiana, which do not have statistical intraspecific differences in genome size (Table S1). One explanation is that these species have a mechanism to maintain genome size constancy, for example, by intraspecific stabilizing selection on genome size . On the other hand, we found obvious intraspecific variation in Lemna minor, Lemna aequinoctialis, Lemna trisulca, and Lemna japonica. Some artifacts of intraspecific variation in genome size have been noted, such as environmentally induced variations, secondary compounds and fluorescence staining inhibitor, and erroneously determined species [15, 40]. However, our experiments are not complicated by these factors. We developed an easy bar-coding method to correctly identify duckweed species, which allowed us to correct any misnamed duckweed in the collection . As cytosolic components may change in response to changes in the environment, we grew the duckweed plants under identical conditions. We used internal standardization such as Brachypodium distachyon, Arabidopsis thaliana, and Physcomitrella patens that were prepared simultaneously and under the same experimental conditions as the duckweed accessions. Both duckweed and the internal standard have very little secondary compounds, which may affect genome size estimates. Additionally, we performed biological replicate on different days to eliminate instrument bias. In addition, intraspecific differences were independently confirmed by simultaneously measuring two accessions of the same species by FCM (Figures 2(b) and 2(c)).
The intraspecific variation may result from different numbers of repeated sequences, including satellite DNA , transposable elements , and ribosomal genes . Large-scale polymorphism of heterochromatic repeats exist in the DNA of Arabidopsis thaliana and could account for about 50% of the variance among the Arabidopsis thaliana accession . In addition, the amount of rDNA accounts for the differences in genome size between closely related lines of Linum usitatissimum (flax) . The activity of transposable elements (TE) potentially multiply 20~100 times (~0.1–1 Mbp) in a single generation . For example, the BARE-1 TE is positively correlated with genome size within wild barley (Hordeum spontaneum) in response to sharp microclimatic divergence . Deletions and insertions (INDELs) are most likely not candidates for genome size differences in duckweed. In Drosophila melanogaster, genome loss is only less than 1 bp per generation , indicating a small contribution to genome-size variation. However, in the fast growing duckweeds, which only need 2~5 days for each generation, one could imagine it is more likely that TE have higher rate than other flowering plants to influence genome size within and between species.
This is the first extensive analysis of genome sizes in duckweeds and examination of genome size variations across a range of taxonomic levels. We showed that duckweeds, in general, have remarkable smaller genome size compared with other flowering plants. The smallest genome size of Spirodela polyrhiza, combined with its sterile and controllable culture, fast growing, and promising application in research, suggest that this species may be good candidates for ongoing whole-genome sequencing projects and a model experimental tool. The 150 Mbp Spirodela polyrhiza genome is being sequenced by the DOE-JGI community-sequencing program (CSP), which will address challenges in alternative energy, bioremediation, and global carbon cycling. Also, the availability of a DNA C-values database of duckweeds and a consensus higher-level phylogenetic tree has opened the way for exploring the general processes underlying the evolution of genomes. Obvious intraspecific variation in duckweeds will also provide nice material to study the mechanism of within-species and between-species variation in genome size. However, the main force driving the intraspecific variance and how the genome size affects the phenotype still requires more research.
We thank Elias Landolt from Institut für Integrative Biologie (Zürich, Switzerland), Lynn Dickey, Nirmala Rajbhandari, and Peaches Staton from BIOLEX (NC, USA), and Judy Acreman UTCC (Toronto, Canada) to generously provided duckweed accessions. We are grateful to Theresa Hyejeong Choi from EOHSI (Rutgers University) for assistance with flow cytometry. We would like to thank Justina Marcinow, Marina Ermakova and Yiheng Yan for experimental help. We thank Julia Trumbull for maintaining the Landolt Duckweed Collection at Rutgers. This work was supported by an Energy Fellowship and a Waksman Fellowship to W. Wang, and startup funds from the Waksman Institute of Microbiology to T. P. Michael.
- D. H. Les, D. J. Crawford, E. Landolt, J. D. Gabel, and R. T. Kimball, “Phylogeny and systematics of Lemnaceae, the duckweed family,” Systematic Botany, vol. 27, no. 2, pp. 221–240, 2002.
- E. Landolt, The Family of Lemnaceae—A Monographic Study, vol. 1, Veroffentlichungen des Geobotanischen Institutes ETH, Stiftung Rubel, Zurich, Switzerland, 1986.
- W. Journey, P. Skillicorn, and W. Spira, Duckweed Aquaculture: A New Aquatic Farming System for Developing Countries, The World Bank, 1993.
- W. S. Hillman, “Calibrating duckweeds: light, clocks, metabolism, flowering,” Science, vol. 193, no. 4252, pp. 453–458, 1976.
- A. M. Stomp, “The duckweeds: a valuable plant for biomanufacturing,” Biotechnology Annual Review, vol. 11, pp. 69–99, 2005.
- W. S. Hillman, “Photoperiodism in Lemna: reversal of night-interruption depends on color of the main photoperiod,” Science, vol. 154, no. 3754, pp. 1360–1362, 1966.
- K. Chaloupkova and C. C. Smart, “The abscisic acid induction of a novel peroxidase is antagonized by cytokinin in Spirodela polyrrhiza L,” Plant Physiology, vol. 105, no. 2, pp. 497–507, 1994.
- R. A. Brain and K. R. Solomon, “A protocol for conducting 7-day daily renewal tests with Lemna gibba,” Nature Protocols, vol. 2, no. 4, pp. 979–987, 2007.
- Y. T. Yamamoto, N. Rajbhandari, X. Lin, B. A. Bergmann, Y. Nishimura, and A. M. Stomp, “Genetic transformation of duckweed Lemna gibba and Lemna minor,” In Vitro Cellular and Developmental Biology—Plant, vol. 37, no. 3, pp. 349–353, 2001.
- N. Ozengin and A. Elmaci, “Performance of duckweed (Lemna minor L.) on different types of wastewater treatment,” Journal of Environmental Biology, vol. 28, no. 2, pp. 307–314, 2007.
- J. J. Cheng and A. M. Stomp, “Growing duckweed to recover nutrients from wastewaters and for production of fuel ethanol and animal feed,” Clean—Soil, Air, Water, vol. 37, no. 1, pp. 17–26, 2009.
- R. Lister, B. D. Gregory, and J. R. Ecker, “Next is now: new technologies for sequencing of genomes, transcriptomes, and beyond,” Current Opinion in Plant Biology, vol. 12, no. 2, pp. 107–118, 2009.
- E. Landolt, The Family of Lemnaceae—A Monographic Study, vol. 2, Veroffentlichungen des Geobotanischen Institutes ETH, Stiftung Rubel, Zurich, Switzerland, 1986.
- G. Geber, Zur Karyosystematik der Lemnaceae, University of Vienna, Vienna, Austria, 1989.
- J. Doležel and J. Bartoš, “Plant DNA flow cytometry and estimation of nuclear genome size,” Annals of Botany, vol. 95, no. 1, pp. 99–110, 2005.
- M. D. Bennett and I. J. Leitch, “Nuclear DNA amounts in angiosperms: progress, problems and prospects,” Annals of Botany, vol. 95, no. 1, pp. 45–90, 2005.
- W. Wang, Y. Wu, Y. Yan, M. Ermakova, R. Kerstetter, and J. Messing, “DNA barcoding of the Lemnaceae, a family of aquatic monocots,” BMC Plant Biology, vol. 10, p. 205, 2010.
- J. Doležel, J. Greilhuber, and J. Suda, “Estimation of nuclear DNA content in plants using flow cytometry,” Nature Protocols, vol. 2, no. 9, pp. 2233–2244, 2007.
- D. W. Galbraith, K. R. Harkins, J. M. Maddox, N. M. Ayres, D. P. Sharma, and E. Firoozabady, “Rapid flow cytometric analysis of the cell cycle in intact plant tissues,” Science, vol. 220, pp. 1049–1051, 1983.
- K. Arumuganathan and E. D. Earle, “Nuclear DNA content of some important plant species,” Plant Molecular Biology Reporter, vol. 9, no. 3, pp. 208–218, 1991.
- G. Schween, G. Gorr, A. Hohe, and R. Reski, “Unique tissue-specific cell cycle in Physcomitrella,” Plant Biology, vol. 5, no. 1, pp. 50–58, 2003.
- J. Doležel, J. Bartoš, H. Voglmayr, J. Greilhuber, and R. A. Thomas, “Nuclear DNA content and genome size of trout and human,” Cytometry A, vol. 51, no. 2, pp. 127–129, 2003.
- E. Landolt, Biosystematic Investigations in the Family of Duckweeds (Lemnaceae), Veroffentlichungen des Geobotanischen Institutes ETH, Stiftung Rubel, Zurich, Switzerland, 1980.
- M. J. Hegarty and S. J. Hiscock, “Genomic clues to the evolutionary success of polyploid plants,” Current Biology, vol. 18, no. 10, pp. R435–R444, 2008.
- C. Seoighe and C. Gehring, “Genome duplication led to highly selective expansion of the Arabidopsis thaliana proteome,” Trends in Genetics, vol. 20, no. 10, pp. 461–464, 2004.
- A. R. Leitch and I. J. Leitch, “Genomic plasticity and the diversity of polyploid plants,” Science, vol. 320, no. 5875, pp. 481–483, 2008.
- K. L. Adams and J. F. Wendel, “Polyploidy and genome evolution in plants,” Current Opinion in Plant Biology, vol. 8, no. 2, pp. 135–141, 2005.
- S. P. Otto, “The evolutionary consequences of polyploidy,” Cell, vol. 131, no. 3, pp. 452–462, 2007.
- M. C. J. Bottini, E. J. Greizerstein, M. B. Aulicino, and L. Poggio, “Relationships among genome size, environmental conditions and geographical distribution in natural populations of NW patagonian species of Berberis L. (Berberidaceae),” Annals of Botany, vol. 86, no. 3, pp. 565–573, 2000.
- D. Ohri and T. N. Khoshoo, “Genome size in gymnosperms,” Plant Systematics and Evolution, vol. 153, no. 1-2, pp. 119–132, 1986.
- A. L. Rayburn and J. A. Auger, “Genome size variation in Zea mays ssp. mays adapted to different altitudes,” Theoretical and Applied Genetics, vol. 79, no. 4, pp. 470–474, 1990.
- M. Ceccarelli, S. Minelli, F. Maggini, and P. G. Cionini, “Genome size variation in Vicia faba,” Heredity, vol. 74, no. 2, pp. 180–187, 1995.
- M. A. Lysak, A. Rostkova, J. M. Dixon, G. Rossi, and J. Dolezel, “Limited genome size variation in Sesleria albicans,” Annals of Botany, vol. 86, pp. 399–403, 2000.
- J. Chrtek Jr., J. Zahradnícek, K. Krak, and J. Fehrer, “Genome size in Hieracium subgenus Hieracium (Asteraceae) is strongly correlated with major phylogenetic groups,” Annals of Botany, vol. 104, no. 1, pp. 161–178, 2009.
- C. A. Knight, N. A. Molinari, and D. A. Petrov, “The large genome constraint hypothesis: evolution, ecology and phenotype,” Annals of Botany, vol. 95, no. 1, pp. 177–190, 2005.
- M. D. Bennett, S. Johnston, G. L. Hodnett, and H. J. Price, “Allium cepa L. cultivars from four continents compared by flow cytometry show nuclear DNA constancy,” Annals of Botany, vol. 85, no. 3, pp. 351–357, 2000.
- J. Greilhuber and R. Obermayer, “Genome size and maturity group in Glycine max (soybean),” Heredity, vol. 78, no. 5, pp. 547–551, 1997.
- B. Hendrix and J. M. Stewart, “Estimation of the nuclear DNA content of Gossypium species,” Annals of Botany, vol. 95, no. 5, pp. 789–797, 2005.
- P. Šmarda, L. Horová, P. Bureš, I. Hralová, and M. Marková, “Stabilizing selection on genome size in a population of Festuca pallens under conditions of intensive intraspecific competition,” New Phytologist, vol. 187, no. 4, pp. 1195–1204, 2010.
- J. Greilhuber, “Intraspecific variation in genome size in angiosperms: identifying its existence,” Annals of Botany, vol. 95, no. 1, pp. 91–98, 2005.
- G. Bosco, P. Campbell, J. T. Leiva-Neto, and T. A. Markow, “Analysis of Drosophila species genome size and satellite DNA content reveals significant differences among strains as well as between species,” Genetics, vol. 177, no. 3, pp. 1277–1290, 2007.
- R. Kalendar, J. Tanskanen, S. Immonen, E. Nevo, and A. H. Schulman, “Genome evolution of wild barley (Hordeum spontaneum) by BARE-1 retrotransposon dynamics in response to sharp microclimatic divergence,” Proceedings of the National Academy of Sciences of the United States of America, vol. 97, no. 12, pp. 6603–6607, 2000.
- C. A. Cullis, “Mechanisms and control of rapid genomic changes in flax,” Annals of Botany, vol. 95, no. 1, pp. 201–206, 2005.
- J. Davison, A. Tyagi, and L. Comai, “Large-scale polymorphism of heterochromatic repeats in the DNA of Arabidopsis thaliana,” BMC Plant Biology, vol. 7, article 44, 2007.
- D. A. Petrov, “Evolution of genome size: new approaches to an old problem,” Trends in Genetics, vol. 17, no. 1, pp. 23–28, 2001.
- D. A. Petrov and D. L. Hartl, “High rate of DNA loss in the Drosophila melanogaster and Drosophila virilis species groups,” Molecular Biology and Evolution, vol. 15, no. 3, pp. 293–302, 1998.