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Journal of Botany
Volume 2011 (2011), Article ID 570319, 9 pages
http://dx.doi.org/10.1155/2011/570319
Research Article

Evolution of Genome Size in Duckweeds (Lemnaceae)

1Rutgers, Department of Plant Biology and Pathology, The Waksman Institute of Microbiology, The State University of New Jersey, Piscataway, NJ 08854, USA
2Monsanto Company, 800 North Lindbergh Boulevard, Creve Coeur, MO 63167, USA

Received 4 February 2011; Revised 5 April 2011; Accepted 19 May 2011

Academic Editor: Johann Greilhuber

Copyright © 2011 Wenqin Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To extensively estimate the DNA content and to provide a basic reference for duckweed genome sequence research, the nuclear DNA content for 115 different accessions of 23 duckweed species was measured by flow cytometry (FCM) stained with propidium iodide as DNA stain. The 1C-value of DNA content in duckweed family varied nearly thirteen-fold, ranging from 150 megabases (Mbp) in Spirodela polyrhiza to 1,881 Mbp in Wolffia arrhiza. There is a continuous increase of DNA content in Spirodela, Landoltia, Lemna, Wolffiella, and Wolffia that parallels a morphological reduction in size. There is a significant intraspecific variation in the genus Lemna. However, no such variation was found in other studied species with multiple accessions of genera Spirodela, Landoltia, Wolffiella, and Wolffia.