Cell Wall Growth and Modulation Dynamics in a Model Unicellular Green Alga—Penium margaritaceum: Live Cell Labeling with Monoclonal Antibodies
Figure 4
Cells incubated in cell wall-degrading enzymes. (a) represents a cell incubated in 200 μL/mL of cellulase for 48 h and then labeled with JIM5. Note the intense labeling (arrows) in the cell center. Scale bar = 7.8 μm. (b) is a magnified view of the JIM5-labeled zone in a cell incubated in cellulase. While the intensity of labeling is greater than surrounding zones, the typical HG lattice is not apparent (arrows). Scale bar = 2.5 μm. (c) shows a predivision phase of cell incubated in cellulase for 72 h. In addition to the intense JIM5 labeling, the morphology of the cell center (arrow) changes forming a small “knob-” like structure. Scale bar = 8.5 μm. (d) highlights the new semicell of a daughter cell after cell division (72 h). Note the knob-like appearance of one pole of the cell and the intense JIM5 labeling (arrows). Scale bar = 3.8 μm. (e) is a profile of a cell incubated for 72 h in 200 μg/mL pectinase. Note the lack of labeling two bands on the cell (arrows). Scale bar = 4.4 μm. (f) is a recently divided cell incubated in pectinase. Note that the HG in the new semicell (arrow) is highly disorganized. Scale bar = 3.9 μm. (g) is a recently divided cell labeled with JIM5 and overlayed with chloroplast scan. Note the lack of labeling in the wall of the new semicell (arrow). Cells could be kept alive for up to two weeks in these enzyme solutions. Scale bar = 7.8 μm.
