Seed Cryopreservation of Some Medicinal Legumes

Table 2

Rate of germination and final germination percentage (G) of Far-Eastern legumes after scarification, cryopreservation (LN), or cryopreservation and scarification (scarification after LN). MC: seed moisture content. L: seed length. SM: seed mass, g per 100 seeds.

Species	Control			Scarification			LN			Scarification after LN			G ± SE, %*	MC, %	L, mm	SM, g
	𝑇0, days	𝑇50, days	G ± S E, %	𝑇0, days	𝑇50, days	G ± SE, %	𝑇0, days	𝑇50, days	G ± SE, %	𝑇0, days	𝑇50, days	G ± SE, %
Astragalus membranaceus	2	11	3.3±1.2	1	2	99.3±1.2	2	11	14.0±3.5	2	2	75.3±6.1	89.3±4.2	6.90±0.18	2.8±0.03	0.4±0.01
Sophora flavescens	7	10	3.0±1.0	3	7	86.8±7.9	4	13	48.9±1.9	4	4	29.9±4.3	78.8±3.3	8.60±0.04	4.86±0.06	4.0±0.18
Trifolium lupinaster	1	7	8.0±2.0	1	2	94.0±5.3	1	3	84.0±2.0	1	2	12.7±1.2	96.7±2.3	7.98±0.75	2.0±0.04	0.33.±0.01
Oxytropis chankaensis	2	6	6.0±2.0	2	2	80.7±8.1	2	6	91.3±4.6	2	2	4.7±1.2	96.0±3.5	7.05±0.34	2.2±0.03	0.24±0.003
O. kamtschatica	2	11	68.0±1.2	2	3	93.6±4.2	2	9	89.3±4.8	—	—	—	—	6.64±0.77	2.26±0.02	0.25±0.005
O. ochotensis	2	12	36.0±1.2	2	2	87.3±5.2	2	9	62.0±3.1	—	—	—	—	7.10±0.18	1.98±0.03	0.2±0.002
O. retusa	6	10	9.0±1.0	2	3	67.0±2.0	4	7	41.3±6.4	—	—	—	—	5.74±0.71	2.68±0.04	0.41±0.003
O. revoluta-1	2	33	42.0±2.0	2	2	79.3±1.9	2	6	84.7±7.1	—	—	—	—	6.68±0.25	1.79±0.03	0.13±0.001
O. revoluta-2	2	25	49.3±4.8	2	2	79.3±3.0	2	7	80.7±2.7	—	—	—	—	6.45±0.40	1.81±0.03	0.13±0.002
Hedysarum austrokurilense	1	8	70.7±1.3	1	2	87.7±4.6	1	7	85.0±1.5	—	—	—	—	—	2.84±0.05	0.47±0.011
H. sachalinense	1	2	85.3±1.8	—	—	—	1	3	73.0±7.5	—	—	—	—	—	4.46±0.10	0.93±0.056
Vicia amurensis	6	11	30.0±4.2	2	3	100	5	11	33.3±2.7	—	—	—	—	—	3.35±0.04	2.47±0.06
V. subrotunda	8	26	5.3±0.7	2	3	74.3±1.8	5	16	53.3±7.1	—	—	—	—	6.97±0.23	3.79±0.07	2.57±0.123

*Summarized germination percentage after cryopreservation (LN) and scarification (scarification after LN).