Genome Mutation Revealed by Artificial Hybridization between Chrysanthemum yoshinaganthum and Chrysanthemum vestitum Assessed by FISH and GISH

Figure 1

(a)–(d). GISH applied on somatic chromosomes in the artificial interspecific F₁ hybrid (2𝑛=45, pentaploid; ID: y-11-4-1-10) between Ch. yoshinaganthum and Ch. vestitum. Biotin-labeled total genomic DNA probe of Ch. vestitum ((a)–(d)) was mixed with approximately 20–40 times blocking DNA of Ch. yoshinaganthum and used. Yellow-colored chromosomes of Ch. vestitum were hybridized with the probe and visualized by FITC, and approximately nine red-colored chromosomes of Ch. yoshinaganthum were not and stained partially red-color by PI. Interphase (a) and prophase ((b)-(c)) show the parental chromosomes separated in two spatial domains without intermix. Bar = 10 μm.

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