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Journal of Botany
Volume 2012 (2012), Article ID 737035, 4 pages
http://dx.doi.org/10.1155/2012/737035
Research Article

Osmotic Stress Induces the Expression of VvMAP Kinase Gene in Grapevine (Vitis vinifera L.)

1Laboratoire de Physiologie Moléculaire des Plantes, Centre de Biotechnologie, B.P. 901, 2050 Hammam Lif, Tunisia
2AlPlanta-Institute for Plant Research, RLP AgroScience GmbH, 67935 Neustadt, Germany

Received 29 July 2011; Revised 14 October 2011; Accepted 3 November 2011

Academic Editor: Andrew Wood

Copyright © 2012 Samia Daldoul et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Abiotic stress adversely affects the growth of grapevine plants. In order to study the early expression changes of genes particularly involved in signal transduction upon salt and drought stresses in grapevines, ESTs derived from a suppressive subtractive hybridization approach (SSH) were selected for expression studies. We were particularly interested in the expression behaviour of the MAP kinase cDNA clone identified by differential screening of the salt-stressed SSH libraries. Interestingly, VvMAP kinase transcript showed a differential expression towards salt and drought treatment in the salt tolerant cultivar Razegui. The upregulation of this transcript was confirmed by RNA blot analysis. Our results revealed that the VvMAP kinase gene could be classified as an osmotic stress responsive gene as its expression was induced by salinity and drought. Furthermore, our study provides the basis for future research on the diverse signaling pathways mediated by MAPKs in grapevine.