Agrobacterium tumefaciens T-DNA Integration and Gene Targeting in Arabidopsis thaliana Non-Homologous End-Joining Mutants
Figure 2
Q-RT-PCR and Western blot analysis. (a) RNA expression of the NHEJ genes AtKu80 (black bars), AtKu70 (white bars) and AtLig4 (grey bars) as determined by Q-RT-PCR, in the Atku70 and Atku80 mutants and wild-type (WT) plants. All sample values were normalized to Roc1 values and were obtained in triplicate. The values of the wild type were set on 1. (b) Western blot analysis of leaf protein samples from the Atku70 and Atku80 mutants and wild-type (WT) plants using anti-AtKu70 antibody (upper panel) and anti-AtKu80 antibody (middle panel), detecting AtKu70 and AtKu80, respectively (arrows). The weak signals of residual AtKu70 in the Atku80 mutant and residual AtKu80 in the Atku70 mutant are indicated with an asterisk. Similar amounts (10 μg) of soluble leaf protein were loaded in each lane as shown by the Coomassie-stained gel (lower panel).
