Agrobacterium tumefaciens T-DNA Integration and Gene Targeting in Arabidopsis thaliana Non-Homologous End-Joining Mutants
Figure 3
Hypersensitivity of Atku70 and Atku80 plants to DNA-damaging treatments. (a) Phenotypes of wild-type (WT) plants and Atku70 and Atku80 mutants after bleomycin or MMS treatment. Four-day-old seedlings were transferred to liquid 1/2MS medium (control) or 1/2MS medium containing different concentrations of bleomycin (bleo) or MMS and were scored 2 weeks after germination. (b) Fresh weight of 2-week-old wild-type plants (black bars) and Atku70 (light grey bars) and Atku80 (dark grey bars) mutants treated with 0.2 μg/mL bleomycin or 0.007% MMS. For each treatment 20 seedlings were weighed in triplicate. Fresh weight of the plants grown for 2 weeks without bleomycin or MMS was set at 1.
