Induction of a bZIP Type Transcription Factor and Amino Acid Catabolism-Related Genes in Soybean Seedling in Response to Starvation Stress
Figure 1
Effects of nutrient starvation on the expression and bZIP53 protein levels of soybean bZIP53 orthologs. (a) A phylogenic tree of bZIP type transcription factors from soybean, cowpea, Arabidopsis, and tobacco was constructed by ClustalW (UPGMA). The genes used were follows: GmbZIP53A and GmbZIP53B (Table 1), VubZIP53 (AB779667), AtbZIP53 (At3g62420), AtbZIP1 (At5g49450), AtGBF1 (At4g36730), AtHY5 (At5g11260), AtFD (At4g35900), AtABI5 (At2g36270), AtAREB1 (At1g45249), and RSG (AB040471). Gene indics of soybean and Arabidopsis were referred to Phytozome V 9.0 (http://www.phytozome.net/) and TGI database (http://compbio.dfci.harvard.edu/tgi/plant.html), respectively. (b) The expression of GmbZIP53A and GmbZIP53B was analyzed by RT-PCR. Actin was used as a standard. (c) Alignments of deduced amino acid sequences of bZIP53 orthologs and other bZIP type transcription factors. A highly conserved amino acid motif (H2N-VEIPEIPDPLLKPWQIPHP-COOH) present in the carboxyl terminal region following bZIP domain was used as an epitope for raising anti-GmbZIP53-specific antibody. (d) Immunoblot of soybean seedlings treated with various nutrient conditions was carried out by anti-GmbZIP53 antibody. Each 50 μg protein per lane was subjected to SDS-PAGE (15% acrylamide gels). Nutrient treatments, semiquantitative RT-PCR, and immunoblot were carried out as described in Section 2.
