Essential Notes Regarding the Design of Functional siRNAs 
       for Efficient Mammalian RNAi

Ui-Tei, Kumiko; Naito, Yuki; Saigo, Kaoru

doi:https://doi.org/10.1155/JBB/2006/65052

BioMed Research International

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RNA Interference

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Review Article | Open Access

Volume 2006 | Article ID 065052 | https://doi.org/10.1155/JBB/2006/65052

Essential Notes Regarding the Design of Functional siRNAs for Efficient Mammalian RNAi

Kumiko Ui-Tei,¹Yuki Naito,²and Kaoru Saigo²

Received25 Jan 2006

Accepted17 Apr 2006

Published29 Jun 2006

Abstract

Short interfering RNAs (siRNAs) are widely used to bring about RNA interference (RNAi) in mammalian cells. Numerous siRNAs may be designed for any target gene though most of which would be incapable of efficiently inducing mammalian RNAi. Certain highly functional siRNAs designed for knockout of a particular gene may render unrelated endogenous genes nonfunctional. These major bottlenecks should be properly eliminated when RNAi technologies are employed for any experiment in mammalian functional genomics. This paper thus presents essential notes and findings regarding the proper choice of siRNA-sequence selection algorithms and web-based online software systems.

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Copyright © 2006 Kumiko Ui-Tei et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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