Development of FRET-Based Assays in the Far-Red Using CdTe Quantum Dots

Chong, E. Z.; Matthews, D. R; Summers, H. D.; Njoh, K. L.; Errington, R. J.; Smith, P. J.

doi:https://doi.org/10.1155/2007/54169

BioMed Research International

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Biomedical Applications of Colloidal Nanocrystals

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Research Article | Open Access

Volume 2007 | Article ID 054169 | https://doi.org/10.1155/2007/54169

Development of FRET-Based Assays in the Far-Red Using CdTe Quantum Dots

E. Z. Chong,¹D. R Matthews,¹H. D. Summers,¹K. L. Njoh,²R. J. Errington,²and P. J. Smith²

Academic Editor: Marek Osinski

Received29 Mar 2007

Accepted11 Oct 2007

Published12 Dec 2007

Abstract

Colloidal quantum dots (QDs) are now commercially available in a biofunctionalized form, and Förster resonance energy transfer (FRET) between bioconjugated dots and fluorophores within the visible range has been observed. We are particularly interested in the far-red region, as from a biological perspective there are benefits in pushing to ∼700 nm to minimize optical absorption (ABS) within tissue and to avoid cell autofluorescence. We report on FRET between streptavidin- (STV-) conjugated CdTe quantum dots, Qdot705-STV, with biotinylated DY731-Bio fluorophores in a donor-acceptor assay. We also highlight the changes in DY731-Bio absorptivity during the streptavidin-biotin binding process which can be attributed to the structural reorientation. For fluorescence beyond 700 nm, different alloy compositions are required for the QD core and these changes directly affect the fluorescence decay dynamics producing a marked biexponential decay with a long-lifetime component in excess of 100 nanoseconds. We compare the influence of the two QD relaxation routes upon FRET dynamics in the presence of DY731-Bio.

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Copyright

Copyright © 2007 E. Z. Chong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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