]>Experimental Study of the Effects of Marrow Mesenchymal Stem Cells Transfected with Hypoxia-Inducible Factor-1<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>α</mml:mi></mml:math> Gene : Figure 2
128627.fig.002a
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128627.fig.002b
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Figure 2: (a) The results of HIF-1 𝛼 -T vector double digestion with EcoR I and Sal I. 1–3: Clones 1–3; m: 𝜆 DNA/Hind III; M:DNA marker; Clones 1 and 2 were positive clone. Red arrow showed the HIF-1 𝛼 band, and yellow arrow showed the T vector band. (b) The results of HIF-1 𝛼 -pcDNA3.1 vector double digestion with Hind III and BamH I. 2-1: HIF-1 𝛼 -pCDNA3.1 positive clone; 2-2: pcDNA3.1 empty vector; M: DL 2000. Red arrow showed the HIF-1 𝛼 band, and black arrow showed the pCDNA3.1 vector band.