Journal of Biomedicine and Biotechnology
Volume 2009 (2009), Article ID 464986, 10 pages
doi:10.1155/2009/464986
Research Article
Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains
1Laboratory of Prokaryotic Enzymes and Metabolites, Centre of Biotechnology of Sfax, Road of Sidi Mansour Km 6, P. O. Box 1177, 3018 Sfax, Tunisia
2Laboratory of Genetic and Microbiology, UMR INRA 1128, IFR 110, Faculty of Sciences and Techniques, University Henri Poincaré, Vandoeuvre-lès-Nancy, 54 003 Nancy Cedex, France
Received 2 December 2008; Accepted 26 March 2009
Academic Editor: Isaac K. O. Cann
Copyright © 2009 Samiha Sioud et al. This is an open access article distributed under the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Linked References
- P. Mazodier, R. Petter, and C. Thompson, “Intergeneric conjugation between Escherichia coli and Streptomyces species,” Journal of Bacteriology, vol. 171, no. 6, pp. 3583–3585, 1989.
- M. Bierman, R. Logan, K. O'Brien, E. T. Seno, R. N. Rao, and B. E. Schoner, “Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp,” Gene, vol. 116, no. 1, pp. 43–49, 1992. View at Publisher · View at Google Scholar
- P. Matsushima and R. H. Baltz, “A gene cloning system for ‘Streptomyces toyocaensis’,” Microbiology, vol. 142, no. 2, pp. 261–267, 1996.
- R. Fouces, M. Rodríguez, E. Mellado, B. Díez, and J. L. Barredo, “Conjugation and transformation of Streptomyces species by tylosin resistance,” FEMS Microbiology Letters, vol. 186, no. 2, pp. 319–325, 2000. View at Publisher · View at Google Scholar
- D. J. MacNeil, J. L. Occi, K. M. Gewain, et al., “Complex organization of the Streptomyces avermitilis genes encoding the avermectin polyketide synthase,” Gene, vol. 115, no. 1-2, pp. 119–125, 1992. View at Publisher · View at Google Scholar
- E. Stegmann, S. Pelzer, K. Wilken, and W. Wohlleben, “Development of three different gene cloning systems for genetic investigation of the new species Amycolatopsis japonicum MG417-CF17, the ethylenediaminedisuccinic acid producer,” Journal of Biotechnology, vol. 92, no. 2, pp. 195–204, 2001. View at Publisher · View at Google Scholar
- T. Voeykova, L. Emelyanova, V. Tabakov, and N. Mkrtumyan, “Transfer of plasmid pTO1 from Escherichia coli to various representatives of the order Actinomycetales by intergeneric conjugation,” FEMS Microbiology Letters, vol. 162, no. 1, pp. 47–52, 1998. View at Publisher · View at Google Scholar
- R. Fouces, M. Rodríguez, E. Mellado, B. Díez, and J. L. Barredo, “Conjugation and transformation of Streptomyces species by tylosin resistance,” FEMS Microbiology Letters, vol. 186, no. 2, pp. 319–325, 2000. View at Publisher · View at Google Scholar
- A. C. Groth, E. C. Olivares, B. Thyagarajan, and M. P. Calos, “A phage integrase directs efficient site-specific integration in human cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 97, no. 11, pp. 5995–6000, 2000. View at Publisher · View at Google Scholar · View at PubMed
- P. Combes, R. Till, S. Bee, and M. C. M. Smith, “The Streptomyces genome contains multiple pseudo-attB sites for the C31-encoded site-specific recombination system,” Journal of Bacteriology, vol. 184, no. 20, pp. 5746–5752, 2002. View at Publisher · View at Google Scholar
- M. A. Gregory, R. Till, and M. C. M. Smith, “Integration site for Streptomyces phage BT1 and development of site-specific integrating vectors,” Journal of Bacteriology, vol. 185, no. 17, pp. 5320–5323, 2003. View at Publisher · View at Google Scholar
- L. Mellouli, R. Ben Ameur-Mehdi, S. Sioud, M. Salem, and S. Bejar, “Isolation, purification and partial characterization of antibacterial activities produced by a newly isolated Streptomyces sp. US24 strain,” Research in Microbiology, vol. 154, no. 5, pp. 345–352, 2003. View at Publisher · View at Google Scholar · View at PubMed
- L. Mellouli, I. Karray-Rebai, S. Sioud, R. Ben Ameur-Mehdi, B. Naili, and S. Bejar, “Efficient transformation procedure of a newly isolated Streptomyces sp. TN58 strain producing antibacterial activities,” Current Microbiology, vol. 49, no. 6, pp. 400–406, 2004. View at Publisher · View at Google Scholar
- R. Ben Ameur-Mehdi, L. Mellouli, F. Chabchoub, S. Fotso, and S. Bejar, “Purification and structure elucidation of two biologically active molecules from a new isolated Streptomyces sp. US 24 strain,” Chemistry of Natural Compounds, vol. 40, no. 5, pp. 510–513, 2004.
- R. Ben Ameur-Mehdi, K. A. Shaaban, I. Karray-Rebai, S. Smaoui, S. Bejar, and L. Mellouli, “Five naturally bioactive molecules including two rhamnopyranoside derivatives isolated from the Streptomyces sp. strain TN58,” Natural Product Research. In press.
- D. Hanahan, “Studies on transformation of Escherichia coli with plasmids,” Journal of Molecular Biology, vol. 166, no. 4, pp. 557–580, 1983. View at Publisher · View at Google Scholar
- K. Srih-Belghith and S. Bejar, “A thermostable glucose isomerase having a relatively low optimum pH: study of activity and molecular cloning of the corresponding gene,” Biotechnology Letters, vol. 20, no. 6, pp. 553–556, 1998. View at Publisher · View at Google Scholar
- M. Mezghani, M. A. Borgi, R. Kammoun, H. Aouissaoui, and S. Bejar, “Construction of new stable strain over-expressing the glucose isomerase of the Streptomyces sp. SK strain,” Enzyme and Microbial Technology, vol. 37, no. 7, pp. 735–738, 2005. View at Publisher · View at Google Scholar
- M. S. B. Paget, L. Chamberlin, A. Atrih, S. J. Foster, and M. J. Buttner, “Evidence that the extracytoplasmic function sigma factor is required for normal cell wall structure in Streptomyces coelicolor A3(2),” Journal of Bacteriology, vol. 181, no. 1, pp. 204–211, 1999.
- J. Sambrook, E. F. Fritsch, and T. Maniatis, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2nd edition, 1989.
- D. A. Hopwood, M. J. Bibb, K. F. Chater, et al., Genetic Manipulation of Streptomyces: A Laboratory Manual, The John Innes Foundation, Norwich, UK, 1985.
- C. J. Thompson, J. M. Ward, and D. A. Hopwood, “DNA cloning in Streptomyces: resistance genes from antibiotic-producing species,” Nature, vol. 286, no. 5772, pp. 525–527, 1980. View at Publisher · View at Google Scholar
- R. H. Baltz, “Genetic recombination by protoplast fusion in Streptomyces,” Developments in Industrial Microbiology, vol. 21, pp. 43–54, 1980.
- F. Flett, V. Mersinias, and C. P. Smith, “High efficiency intergeneric conjugal transfer of plasmid DNA from Escherichia coli to methyl DNA-restricting streptomycetes,” FEMS Microbiology Letters, vol. 155, no. 2, pp. 223–229, 1997. View at Publisher · View at Google Scholar
- T. Kieser, M. J. Bibb, M. J. Bittner, K. F. Chater, and D. A. Hopwood, Practical Streptomyces Genetics: A Laboratory Manual, The John Innes Foundation, Norwich, UK, 2000.
- T. E. Maniatis, E. F. Fritsch, and J. Sambrook, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA, 1982.
- F. Sanger, S. Nicklen, and A. R. Coulson, “DNA sequencing with chain-terminating inhibitors,” Proceedings of the National Academy of Sciences of the United States of America, vol. 74, no. 12, pp. 5463–5467, 1977. View at Publisher · View at Google Scholar
- S. F. Altschul, T. L. Madden, A. A. Schäffer, et al., “Gapped BLAST and PSI-BLAST: a new generation of protein database search programs,” Nucleic Acids Research, vol. 25, no. 17, pp. 3389–3402, 1997. View at Publisher · View at Google Scholar
- M. J. Bibb, G. R. Janssen, and J. M. Ward, “Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus,” Gene, vol. 38, no. 1–3, pp. 215–226, 1985. View at Publisher · View at Google Scholar
- M. M. Bradford, “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding,” Analytical Biochemistry, vol. 72, no. 1-2, pp. 248–254, 1976. View at Publisher · View at Google Scholar
- J. Nikodinovic, K. D. Barrow, and J.-A. Chuck, “High frequency transformation of the Amphotericin-producing bacterium Streptomyces nodosus,” Journal of Microbiological Methods, vol. 55, no. 1, pp. 273–277, 2003. View at Publisher · View at Google Scholar
- S.-U. Choi, C.-K. Lee, Y.-I. Hwang, H. Kinoshita, and T. Nihira, “Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Kitasatospora setae, a bafilomycin producer,” Archives of Microbiology, vol. 181, no. 4, pp. 294–298, 2004. View at Publisher · View at Google Scholar · View at PubMed
- S. Kuhstoss and R. N. Rao, “Analysis of the integration function of the streptomycete bacteriophage C31,” Journal of Molecular Biology, vol. 222, no. 4, pp. 897–908, 1991. View at Publisher · View at Google Scholar
- R. H. Baltz, “Genetic manipulation of antibiotic-producing Streptomyces,” Trends in Microbiology, vol. 6, no. 2, pp. 76–83, 1998. View at Publisher · View at Google Scholar
- A. N. Luzhetskii, B. E. Ostash, and V. A. Fedorenko, “Intergeneric conjugation Escherichia coli—Streptomyces globisporus 1912 using integrative plasmid pSET152 and its derivatives,” Russian Journal of Genetics, vol. 37, no. 10, pp. 1123–1129, 2001. View at Publisher · View at Google Scholar
- S. H. Bhosale, M. B. Rao, and V. V. Deshpande, “Molecular and industrial aspects of glucose isomerase,” Microbiological Reviews, vol. 60, no. 2, pp. 280–300, 1996.