Journal of Biomedicine and Biotechnology
Volume 2009 (2009), Article ID 637942, 8 pages
doi:10.1155/2009/637942
Research Article

Nonviral Production of Human Interleukin-7 in Spodoptera Frugiperda Insect Cells as a Soluble Recombinant Protein

1Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, and Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, 3775 University Street, Montreal, PQ, H3A 2B4, Canada
2Biotechnology Research Institute (BRI), National Research Council (NRC), 6100 Royalmount Avenue, Montreal, PQ, H4P 2R2, Canada

Received 17 January 2008; Revised 14 May 2008; Accepted 7 August 2008

Academic Editor: Gerald Schumann

Copyright © 2009 Maryam Mirzaei et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. F. Altmann, E. Staudacher, I. B. H. Wilson, and L. März, “Insect cells as hosts for the expression of recombinant glycoproteins,” Glycoconjugate Journal, vol. 16, no. 2, pp. 109–123, 1999. View at Publisher · View at Google Scholar
  2. L. K. Miller, “Baculoviruses as gene expression vectors,” Annual Review of Microbiology, vol. 42, pp. 177–199, 1988. View at Publisher · View at Google Scholar · View at PubMed
  3. D. J. Fitzgerald, P. Berger, C. Schaffitzel, K. Yamada, T. J. Richmond, and I. Berger, “Protein complex expression by using multigene baculoviral vectors,” Nature Methods, vol. 3, no. 12, pp. 1021–1032, 2006. View at Publisher · View at Google Scholar · View at PubMed
  4. E. Honjo, Y. Shoyama, T. Tamada, et al., “Expression of the extracellular region of the human interleukin-4 receptor α chain and interleukin-13 receptor α1 chain by a silkworm-baculovirus system,” Protein Expression and Purification, vol. 60, no. 1, pp. 25–30, 2008. View at Publisher · View at Google Scholar · View at PubMed
  5. H. P. Lesch, S. Turpeinen, E. A. Niskanen, A. J. Mähönen, K. J. Airenne, and S. Ylä-Herttuala, “Generation of lentivirus vectors using recombinant baculoviruses,” Gene Therapy, vol. 15, no. 18, pp. 1280–1286, 2008.
  6. M. D. Summers, “Milestones leading to the genetic engineering of baculoviruses as expression vector systems and viral pesticides,” Advances in Virus Research, vol. 68, pp. 3–73, 2006. View at Publisher · View at Google Scholar · View at PubMed
  7. P. J. Farrell, M. Lu, J. Prevost, C. Brown, L. Behie, and K. Iatrou, “High-level expression of secreted glycoproteins in transformed lepidopteran insect cells using a novel expression vector,” Biotechnology and Bioengineering, vol. 60, no. 6, pp. 656–663, 1998. View at Publisher · View at Google Scholar
  8. M. J. Fraser, “Expression of eucaryotic genes in insect cell cultures,” In Vitro Cellular & Developmental Biology—Plant, vol. 25, no. 3, pp. 225–235, 1989. View at Publisher · View at Google Scholar
  9. J. Benting, S. Lecat, D. Zacchetti, and K. Simons, “Protein expression in Drosophila Schneider cells,” Analytical Biochemistry, vol. 278, no. 1, pp. 59–68, 2000. View at Publisher · View at Google Scholar · View at PubMed
  10. A. R. Bernard, T. A. Kost, L. Overton, et al., “Recombinant protein expression in a Drosophila cell line: comparison with the baculovirus system,” Cytotechnology, vol. 15, no. 1–3, pp. 139–144, 1994. View at Publisher · View at Google Scholar
  11. T. A. Pfeifer, “Expression of heterologous proteins in stable insect cell culture,” Current Opinion in Biotechnology, vol. 9, no. 5, pp. 518–521, 1998. View at Publisher · View at Google Scholar
  12. Ö. Alpdogan and M. R. M. van den Brink, “IL-7 and IL-15: therapeutic cytokines for immunodeficiency,” Trends in Immunology, vol. 26, no. 1, pp. 56–64, 2005. View at Publisher · View at Google Scholar · View at PubMed
  13. R. G. Goodwin, S. Lupton, A. Schmierer, et al., “Human interleukin 7: molecular cloning and growth factor activity on human and murine B-lineage cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 86, no. 1, pp. 302–306, 1989. View at Publisher · View at Google Scholar
  14. Q. Jiang, W. Q. Li, F. B. Aiello, et al., “Cell biology of IL-7, a key lymphotrophin,” Cytokine & Growth Factor Reviews, vol. 16, no. 4-5, pp. 513–533, 2005. View at Publisher · View at Google Scholar · View at PubMed
  15. P. A. Welch, A. E. Namen, R. G. Goodwin, R. Armitage, and M. D. Cooper, “Human IL-7: a novel T cell growth factor,” The Journal of Immunology, vol. 143, no. 11, pp. 3562–3567, 1989.
  16. M. B. Widmer, P. J. Morrissey, R. G. Goodwin, et al., “Lymphopoiesis and IL-7,” International Journal of Cell Cloning, vol. 8, pp. 168–170, 1990, discussion 171-172.
  17. P. J. Farrell, L. A. Behie, and K. Iatrou, “Secretion of cytoplasmic and nuclear proteins from animal cells using novel secretion modules,” Proteins: Structure, Function and Genetics, vol. 41, no. 1, pp. 144–153, 2000. View at Publisher · View at Google Scholar
  18. P. J. Farrell, L. A. Behie, and K. Iatrou, “Transformed lepidopteran insect cells: new sources of recombinant human tissue plasminogen activator,” Biotechnology and Bioengineering, vol. 64, no. 4, pp. 426–433, 1999. View at Publisher · View at Google Scholar
  19. T. A. Pfeifer, D. D. Hegedus, T. A. Grigliatti, and D. A. Theilmann, “Baculovirus immediate-early promoter-mediated expression of the ZeocinTM resistance gene for use as a dominant selectable marker in Dipteran and Lepidopteran insect cell lines,” Gene, vol. 188, no. 2, pp. 183–190, 1997. View at Publisher · View at Google Scholar
  20. D. A. Theilmann and S. Stewart, “Molecular analysis of the trans-activating IE-2 gene of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus,” Virology, vol. 187, no. 1, pp. 84–96, 1992. View at Publisher · View at Google Scholar
  21. M. Mirzaei, B. Jardin, C. B. Elias, and S. Prakash, “Expression and production of human interleukin-7 in insect cells using baculovirus expression vector system (BEVS),” Applied Biochemistry and Biotechnology. In press. View at Publisher · View at Google Scholar · View at PubMed
  22. D. A. Theilmann and S. Stewart, “Tandemly repeated sequence at the 3 end of the IE-2 gene of the baculovirus Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus is an enhancer element,” Virology, vol. 187, no. 1, pp. 97–106, 1992. View at Publisher · View at Google Scholar
  23. L. K. Miller, “Baculoviruses for foreign gene expression in insect cells,” Biotechnology, vol. 10, pp. 457–465, 1988.