Journal of Biomedicine and Biotechnology
Volume 2009 (2009), Article ID 789526, 11 pages
doi:10.1155/2009/789526
Research Article

High Harvest Yield, High Expansion, and Phenotype Stability of CD146 Mesenchymal Stromal Cells from Whole Primitive Human Umbilical Cord Tissue

Rebecca C. Schugar,1,2 Steven M. Chirieleison,1 Kristin E. Wescoe,1,3 Benjamin T. Schmidt,1,3 Yuko Askew,1 Jordan J. Nance,1 Joshua M. Evron,1 Bruno Peault,4,5 and Bridget M. Deasy1,3,5,6

1Stem Cell Research Center, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USA
2Center for Cardiovascular Research, Washington University School of Medicine, St. Louis, MO 63110, USA
3Department of Bioengineering, School of Engineering, University of Pittsburgh, Pittsburgh, PA 15219, USA
4David Geffen School of Medicine, University of California-Los Angeles, LA 90095, USA
5McGowan Institute for Regenerative Medicine, Pittsburgh, PA 15219, USA
6Department of Orthopedic Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15260, USA

Received 9 June 2009; Accepted 11 September 2009

Academic Editor: Brynn Levy

Copyright © 2009 Rebecca C. Schugar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Online Supplemental Material includes in depth characterization of UC physical measurements (Table 1). Additional histological images and characterization of DNA content is included in Supplemental Figure 1. MSC marker analysis and growth rates of dispase‐isolated cells through 10 passages is shown in Supplemental Figure 2. Multipotent differentiation of UC-derived cell populations is shown in Supplemental Figure 3. Finally, MSC marker analysis on UCSCs grown in EGM2 and DMEM is illustrated in Supplemental Figure 4.

  1. Legends for Supplementary Material
  2. Supplementary Table
  3. Supplementary Figure 1
  4. Supplementary Figure 2
  5. Supplementary Figure 3
  6. Supplementary Figure 4