Isolation of Specific Clones from Nonarrayed BAC Libraries through Homologous Recombination
Figure 4
PCR analysis of 7 BAC clones before and after targeting. Kanamycin-resistant clones (lanes 1–7) were found by targeted recombination screening of the library. The corresponding “original” clones (“1–7 k” lanes) were identified from the arrayed library by radioactive probe screening and served as a control. All clones were screened with gene-specific primers flanking the putative integration site. Lanes 1 through 7 show candidate BACs identified for EMS1, MGC, SKB1, GMPPA, FLJ20539, NFAT5, and GRIA3, respectively. As anticipated, in all seven cases, the size of PCR product was larger than 1.3-Kb compared to the alleged original clone.
