BioMed Research International

Research Article

Isolation of Specific Clones from Nonarrayed BAC Libraries through Homologous Recombination

Table 2

PCR analysis of nonarrayed library amplification.
GenePosition in human genomePCR results
Before amplificationAfter amplification
No. of cells per PCRNo. of cells per PCR
2 1032 1041042 1032 104104
EMS1chr11:69922297–69960337−+++++
MGC13034chr5:72504796–72506724−+++++
SKB1chr14:22459578–22468422−++−++
GMPPAchr2:220190181–220197215−++−++
FLJ20539chr11:60451115–60458665−++−++
NFAT5chr16:68157387–68288860−++−++
GRIA3chrX:122043692–122348328−++−++
DKFZP564D166chr17:58852873–58855920−++−++
IDAXchr4:105750948–105770071−++−++
HBBchr11:5,246,696–5,248,301−++−++