Association between a Tetranucleotide Repeat Polymorphism of SPAG16 Gene and Cataract in Male Children

Figure 3

Structure analysis of transcripts containing different number of tetra repeats of D2S2944 marker. MFOLD predicted stem-loop structure depending on the number of (GATA)_𝑛 repeats in the microsatellite sequence of D2S2944 (GenBank: G08147.1) present in the intron 10 of SPAG16 gene. Energetically more favorable stem-loop structure are formed in presence of >(GATA)₁₃ repeats.

(a)  (GATA)₀₉ repeat containing motif: 25th-61st nucleotide

(b) (GATA)₁₂ repeat containing motif: 25th-73rd nucleotide

(c) (GATA)₁₅ repeat containing motif: 25th-85th nucleotide of the PCR product sequence as in GenBank: G08147.1