Cell Volume and Sodium Content in Rat Kidney Collecting Duct Principal Cells During Hypotonic Shock

Figure 3

Synchronized time courses of cell volume and $[{\text{Na}}^{+}{]}_{\text{i}}$ in rat kidney OMCD principal cells. $▼$: gradient signal from the valve: switch from normal PBS $[{\text{Na}}^{+}{]}_{\text{i}}$ 280 mM to hypotonic 0.5 PBS $[{\text{Na}}^{+}{]}_{\text{i}}$ 140 mM. Absciss: time (seconds). Ordinate: ($•$) cell volume ($\mu$m³). ($■$) intracellular sodium concentration $[{\text{Na}}^{+}{]}_{\text{i}}$ (mM). ($▲$) calculated content of intracellular sodium ($\times 10$ fmol/cell).