Cell Volume and Sodium Content in Rat Kidney Collecting Duct Principal Cells During Hypotonic Shock

Figure 4

Effect of hypotonic medium on cell volume of OMCD principal cells (representative curve). Cell volumes were estimated according calcein fluorescence intensity. $▼$: gradient signal from the valve: switch from normal PBS $[{\text{Na}}^{+}{]}_{\text{i}}$ 280 mM to hypotonic 0.5 PBS $[{\text{Na}}^{+}{]}_{\text{i}}$ 140 mM, and back to normal PBS. (1) control normal PBS $[{\text{Na}}^{+}{]}_{\text{i}}$ 280 mM. (2) peak of cell volume during swelling. (3) steady state in hypotonic 0.5 PBS $[{\text{Na}}^{+}{]}_{\text{i}}$ 140 mM.