Selective Detection of NADPH Oxidase in Polymorphonuclear Cells by Means of NAD(P)H-Based Fluorescence Lifetime Imaging
Figure 1
(a)
Time series of 2D fluorescence images obtained by means of steady-state TPLSM
representing a neutrophil granulocyte (PMN) phagocyting one of three conidia of
Aspergillus fumigatus. The
fluorescence of the CTO stained PMN (red) was observed at 590 nm, while that of
the FITC stained fungus (green) was observed at 535 nm. (, time step = 30 seconds, time lapse = 10 minutes)
(b) 3D fluorescence image
of a PMN and phagocytosed conidia. TPLSM results reveal that the fungal spores
are readily internalised by the PMNs. The -step between two consecutive images
is 1 m.
