Selective Detection of NADPH Oxidase in Polymorphonuclear Cells by Means of NAD(P)H-Based Fluorescence Lifetime Imaging
Figure 3
(a)
Fluorescence lifetime images of free ( image) and enzyme-bound NAD(P)H ( image), respectively, as they resulted from
the biexponential FLIM experiments performed on PMNs suspended in PBS + 1% FCS.
The distributions of and , respectively, in these images are shown in
the left diagram. The fluorescence lifetime images were obtained by overlapping
intensity images in gray scale with the spatially resolved fluorescence
lifetime information in colour scale. (b) image (green)
and image (red) obtained in FLIM experiments on a
mixed suspension of PMNs and aspergillus conidia, as well as, the overlapp of
the and images. The overlapped image confirms that
conidia contain only free NAD(P)H, that is, their metabolic activity is very
low, whereas PMNs contain both free and enzyme-bound NAD(P)H.
