]>Tropomyosin Period 3 Is Essential for Enhancement of Isometric Tension in Thin Filament-Reconstituted Bovine Myocardium : Figure 11
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Figure 11: SDS-PAGE of reconstituted myocardium with gradient gel. Lane 1: native myocardium. Lane 2: gelsolin-treated myocardium. Lane 3: actin-filament reconstituted myocardium. Lane 4: purified nfTm. Lane 5: myocardium reconstituted with nfTm and Tn after actin-filament reconstitution. Lane 6: purified ▵ 2 T m . Lane 7: myocardium reconstituted with ▵ 2 T m and Tn after actin-filament reconstitution. Lane 8: purified ▵ 3 T m . Lane 9: myocardium reconstituted with ▵ 3 T m and Tn after actin-filament reconstitution. Lane 10: molecular weight (MW) markers as labeled. Lanes 1, 4 and 5: same gel. Lanes 6 and 7: same gel. Lanes 8, 9 and 10: same gel. 𝛼 - 𝐴 𝑐 = 𝛼 -actinin, Ge = gelsolin. Gelsolin is the band just below the 𝛼 - actinin band in lane 2. ▵ 2 T m and ▵ 3 T m run at MW ∼ 3 0  kD, below the nfTm and native Tm, which run at MW ∼ 3 4 kD.