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Table 5: Kinetic constants of Scheme 1. *P value ≤.05 compared to nfTm; **P value ≤.01 compared to nfTm; else, P value >.05. The kinetic constants (for definitions, see Scheme 1) were measured by the sinusoidal length perturbation method at 25°C, ionic strength 200 mM, and pH 7.00. K2 = k2/k−2 and K4 = k4/ k−4. The data were individually fitted to the respective equations, averaged, and tabulated with ±SEM. The number of experiments is given in parentheses ( ). †Data based on Lu et al. [13].

nfTm Acetyl Tm† Units 6.9 ± 1.8 (13) 6.6 ± 1.8 (7) 4.6 ± 2.4 (10) 6.2 ± 1.3 mM-1 2.3 ± 1.0 (13) 1.7 ± 0.5 (7) 1.0 ± 0.4 (10) 1.08 ± 0.21 — 0.44 ± 0.08 (10) 0.61 ± 0.11 (13) 1.52 ± 0.54 0.61 ± 0.24 — 0.13 ± 0.03 (10) 0.30 ± 0.07 0.68 ± 0.21 0.11 ± 0.03 mM-1 15 ± 2 (13) 17 ± 2 (7) 14 ± 3 (10) 22 ± 4 13 ± 2 (13) 14 ± 3 (7) 22 ± 3 21 ± 2 3.5 ± 0.3 (10) 2.8 ± 0.3 (13) 2.8 ± 0.4 (10) 5.2 ± 1.1 14.1 ± 5.8 (10) 6.0 ± 1.0 (13) 3.8 ± 1.1 14.5 ± 3.4