Crystal Structural and Functional Analysis of the Putative Dipeptidase from Pyrococcus horikoshii OT3

Table 3

Effect of metal ions on dipeptidase (Met-Pro) activity of Ph dpd and Zn-Phdpd.

Metal (1.2 mM)	Relative activity (%)
	Phdpd	Zn-Phdpd
CoCl2	100	100
MnCl2	155	53
ZnCl2	1	1
no metal	20	1

Zn-Ph dpd was not inhibited in the presence of cacodylate ion from 0.4 𝜇M to 40 mM concentrations.
Specific activity of Ph dpd in the presence of 1.2 mM Co ions was 3 times that of Zn-Phdpd.