Exploring the Membrane Mechanism of the Bioactive Peptaibol Ampullosporin A Using Lipid Monolayers and Supported Biomimetic Membranes

Table 1

Optical thickness measured by SPR after the interaction of AmpA at increasing concentrations with the biomimetic lipid membranes.

Peptide layer thickness (Å)^(a)

0.5 μM

1 μM

2 μM

5 μM

8 μM

10 μM

Before

After

Before

After

Before

After

Before

After

Before

After

Before

After

HBM

0

0

0.5±0.5

0

0.5

0

2±0.5

0

2.5±0.5

0

2.5±0.5

0

𝑡-BLM

0

0

1.5±0.5

0

3±0.4

0

5±0.4

1±0.5

—

Membrane disruption

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Membrane disruption

^(a)The baseline was attributed to the lipid membrane thickness before peptide injections. The thickness values are given before and after the buffer rinsing step annotated 𝑅 in Figure 4.