Examinations of tRNA Range of Motion Using Simulations of Cryo-EM Microscopy and X-Ray Data
Figure 4
Cryogenic-electron microscopy fitting using Maxwell’s demon Molecular Dynamics for tRNAs A/T, A, P, and P/E is shown. All tRNA atomic models are shown in ribbons/CPK, while densities are as solid. (a) A/T-site structure for tRNA fit to density, cross-correlation coefficient (CCC) of 95% between the structures theoretical density and the cryo-EM experimental density (Divergent stereo). (b) A-site structure for tRNA fit to density with a CCC of 83% (Divergent stereo). (c) P-site structure for tRNA with a CCC of 86% (Divergent stereo). (d) P/E-site structure with a CCC >90% (Divergent stereo).
