pH-Dependent Interaction between C-Peptide and Phospholipid Bicelles

Figure 5

(a) 2D TOCSY spectra of 200 𝜇M C-peptide in buffer (black) and in DMPC/DHPC bicelles (300 mM total lipid, 𝑞=0.25) (red) at pH 3.2. (b) Differences in amide proton chemical shifts between C-peptide in citrate buffer and C-peptide in DMPC/DHPC bicelles at pH 3.2 are plotted against differences in α proton chemical shifts (i.e., chemical shift in bicelles and chemical shift in buffer).