Analysis of the REJ Module of Polycystin-1 Using Molecular Modeling and Force-Spectroscopy Techniques

Table 1

REJ constructs used for SMFS experiments.

Protein construct	Amino acid; (human PC1) Genbank no. L33243	Expression system	Remarks	Expression vector
REJd1	2151–2256	Insect cell Sf9	Very low expression/insoluble	pFastBac
REJd4	2468–2575	Insect cell Sf9	Very low expression/insoluble	pFastBac
REJd1-4	2151–2575	Insect cell Sf9	Very low expression/insoluble	pVL1392
MBP-REJd1-I27	2151–2256	E. coli	Good expression/soluble	p202
MBP-REJd1,2-I27	2151–2375	E. coli	Good expression/soluble	p202
MBP-REJd3,4-I27	2380–2575	E. coli	Very low expression/insoluble	p202
(I27)3-REJd3,4-(I27)2	2380–2575	E. coli/Sf9	Good expression/soluble	pRSETA/pVL1392
(I27)3-REJd4-(I27)2	2468–2575	E. coli/Sf9	Good expression/soluble	pRSETA/pVL1392

MBP: maltose-binding protein; I27: titin domain I27.