Potassium Current Is Not Affected by Long-Term Exposure to Ghrelin or GHRP-6 in Somatotropes GC Cells
Figure 2
Components of the voltage-dependent potassium current in the cellular line of rat GC somatotropes. (a) Family of representative traces of the voltage-dependent K+ current in the GC cells evoked by depolarizing voltage pulses lasting 1.5 sec, with increases of 10 mV starting from a prepulse of 500 ms and a holding potential of −130 mV, the initial holding potential have been fixed at −80 mV. The lower part shows the acquisition protocol. (b) Representative traces of the voltage-dependent K+ current evoked by 1.5 s depolarizing pulses, starting from a prepulse lasting 500 ms and a holding potential of −40 mV, increasing by 10 mV at a time to 60 mV; the pulse protocol is shown in the lower part. (c) Transient K+ current isolated through a point-by-point subtraction of currents from traces (a) and (b): . (d) Current-voltage relationship at peak of current; transient component (; the first 50 ms), and sustained component (; 1.455–1.5 s). (e) Current-voltage relationship of the point-by-point subtraction between (a) and (b): the measurement was taken in the first 50 ms .
