Validated Spectrophotometric Methods for Simultaneous Determination of Food Colorants and Sweeteners

Table 1

Statistical parameters of the simultaneous determination of colorants (IND, BB) and sweeteners (ACE-K, ) by ZCDS and RDS methods.

Methods	Analyte	Selected Wavelength (nm)	Concentration range; (µgmL^−1)	Regression equations^a	Correlation coefficient; (𝑟)	Detection Limit (µgmL^−1)	Quantification Limit; (µgmL^−1)
ZCDS	IND	1D348	2–10	4.53⋅10^−4 C* − 1.24⋅10^−4	0.9998	0.33	0.47
	BB	^1D420	2–10	6.39⋅10^−4 C* − 6.2⋅10^−5	0.9999	0.18	0.34
	ACE-K	^1D242	2–10	1.75⋅10^−3 C*	0.9999	0.04	0.10
	ASP	^3D227	2–10	2.75⋅10^−5 C* − 3⋅10^−6	0.9995	0.18	0.38
RDS	IND	^1D356	2–10	0.074 C* + 4.7⋅10^−3	0.9994	0.33	0.44
	BB	^1D419	2–10	0.055 C* + 6.95⋅10^−3	0.9992	0.14	0.22
	ACE-K	^1D248	2–10	1.01 C* + 0.071	0.9998	0.07	0.08
	ASP	^3D225	2–10	2.02⋅10^−5 C* − 1⋅10^−6	0.9999	0.05	0.06

^aFive separate determinations were performed and mean calculated.
*C is the concentration of the analyte (µg mL⁻¹).