Review Article

An Overview of Analytical Determination of Diltiazem, Cimetidine, Ranitidine, and Famotidine by UV Spectrophotometry and HPLC Technique

Table 4

Application of HPLC to the simultaneous determination of H2 receptor antagonists.

ColumnMobile phaseDetection (nm)Ref.

RP-mediterranea column C18 (250 × 4.6 mm, 5 μm)ACN : methanol : water (20 : 28 : 52 v/v/v) pH 2.8 adjusted by phosphoric acid221, 256 and 267[211]
Hypersil, ODS, C18 (150 9 4.6 mm, 5 lm) and Purospher_STAR RP-18 columnACN : water (70 : 25 v/v) pH adjusted to 3.0 by phosphoric acid225 [212]
Purospher star C18 (5 μm, 25 × 0.46 cm) columnmethanol : water (60 : 40 v/v), pH adjusted to 3.0 ± 0.02 by phosphoric acid225[213]
Purospher Star RP18 endcapped (250 mm × 4.6 mm I.D.) column packed with 5-μm particles.Methanol-water-triethylamine
(20 : 80 : 0.05), whose pH was adjusted to 3.0 with phosphoric acid
229[214]
Nucleosil, 100-10, C-18 column (250 × 4.6 mm, 10 micron)Methanol : water : acetonitrile (60 : 45 : 5 v/v, pH 3.83)240[215]
Purospher STAR C18 (250 × 4.6 mm, 5 μm) columnMethanol : water : ACN (54 : 41 : 5 v/v/v) pH 2.7 adjusted by phosphoric acid232 and 250[216]
(15 cm × 4.6 mm ID) of Inertsil ODS-2 (5 μm) column0.04 M aqueous sodium dihydrogen phosphate : ACN : methanol : TEA (345 : 20 : 35 : 0.7 v/v/v/v)230 nm[217]