High-Performance Liquid Chromatographic Ultraviolet Determination of Memantine Hydrochloride after In Vitro Transdermal Diffusion Studies

Table 1

Optimization of the memantine extraction process.

Condition		Volume modification (𝜇L)	Real concentration (𝜇g/mL)	Concentration recovered (%)	R.S.D. (%)
Solvent extraction	Sample	180	20.0	102	0.500
		200	25.0	100	1.30
		220	31.4	96.5	8.64
	ACN	160	30.0	101	8.32
		180	25.0	100	1.30
		220	21.4	94.7	0.84
	KOH	180	25.0	99.6	4.60
		200	25.0	100	1.30
		220	25.0	98.4	1.48