Validation of Quetiapine Fumarate in Pharmaceutical Dosage by Reverse-Phase HPLC with Internal Standard Method

Table 9

Effect of various parameters in assessment of method.

Parameters	Variation	Observed values
		R.T	T.P	Tailing	Resolution
Flow rate	0.5 mL/min	3.63	5942	1.22	10.86
	0.7 mL/min	3.62	5910	1.19	10.23
Column temperature	20°C	3.62	6308	1.25	11.22
	30°C	3.75	6115	1.22	9.79
Mobile phase	90% organic	3.63	5960	1.23	10.79
	110% organic	3.62	5840	1.25	9.29