RP-HPLC Analytical Method Development and Validation for Simultaneous Estimation of Three Drugs: Glimepiride, Pioglitazone, and Metformin and Its Pharmaceutical Dosage Forms

Table 1

System suitability results.

Standard	Average	% RSD
Glimepiride
Retention time	5.043	0.26
Area	14711933	0.13
Resolution	4.924
Theoretical plates	7063
Asymmetry	0.898
Pioglitazone
Retention time	3.977	0.27
Area	11302936	0.1
Resolution	15.52
Theoretical plates	6782
Asymmetry	0.94
Metformin
Retention time	1.342	0
Area	11142195	0.31
Resolution	0
Theoretical plates	1347
Asymmetry	1.17
Dosage form
Glimepiride
Retention time	5.071	0.11
Area	14719434	0.1
Resolution	4.99
Theoretical plates	7142.925
Asymmetry	0.88
Pioglitazone
Retention time	3.992	0
Area	11285309.5	0.11
Resolution	15.775
Theoretical plates	6893.845
Asymmetry	0.93
Metformin
Retention time	1.342	0
Area	11109070.5	0.31
Resolution	0
Theoretical plates	1396.245
Asymmetry	1.205