RP-HPLC Analytical Method Development and Validation for Simultaneous Estimation of Three Drugs: Glimepiride, Pioglitazone, and Metformin and Its Pharmaceutical Dosage Forms

Table 3

Results for accuracy of the method.

		Initial conc area	Sol 1 area	50% area	Sol 1—50% area	% Recovery
Sol 1	Glimepiride	14657264	22563825	8023992	14539833	99.2
	Pioglitazone	11302419	17393625	6189621	11204004	99.13
	Metformin	11072363	20056814	9030106	11026708	99.59
		Initial conc area	Sol 2 area	75% area	Sol 2—75% area	% Recovery
Sol 2	Glimepiride	14657264	26129857	11630341	14499516	98.92
	Pioglitazone	11302419	20320163	8954005	11366158	100.56
	Metformin	11072363	21365397	10146889	11218508	101.32
		Initial conc area	Sol 3 area	100% area	Sol 3—100% area	% Recovery
Sol 3	Glimepiride	14657264	29367481	14657264	14710217	100.36
	Pioglitazone	11302419	22539487	11302419	11237068	99.42
	Metformin	11072363	22249678	11072363	11177315	100.95
		Initial conc area	Sol 4 area	125% area	Sol 4—125% area	% Recovery
Sol 4	Glimepiride	14657264	32182741	17567370	14615371	99.71
	Pioglitazone	11302419	28483584	13618562	11225022	99.32
	Metformin	11072363	22950236	11878593	11071643	99.99
		Initial conc area	Sol 5 area	150% area	Sol 5—150%	% Recovery
Sol 5	Glimepiride	14657264	34362870	19549044	14813826	101.07
	Pioglitazone	11302419	26320185	15120715	11199470	99.09
	Metformin	11072363	23472537	12416531	11056006	99.85