Separation and Identification of Furocoumarin in Fruits of Heracleum candicans DC. by HPTLC

Table 1

Method validation parameters for the estimation of psoralen and heraclenol by HPTLC densitometry.

Parameter	Results
	Psoralen (1)	Heraclenol (2)
Accuracy (%)	85.64	93.51
Precision (% RSD)
CV (instrument precision)	0.037948
CV (method precision)	1.31	1.14
Interday precision	0.73	0.80
Intraday precision	0.68	0.75
Limit of detection	0.86 µg	2.79 µg
Limit of quantification	2.62 µg	4.82 µg
Specificity	Specific	Specific
Correlation coefficient	0.973	0.964
Linearity range	1–5 µg spot^−1	1–4 µg spot^−1
Linear regression coefficient	𝑦=4.010𝑥+8878	𝑦=1.253𝑥+1790
Slope	4.010±0.4694	1.254±0.1698
𝑌-intercept	8878±1285	1791±465.1
𝑃 value	0.0134	0.0179