Research Article
Separation and Identification of Furocoumarin in Fruits of Heracleum candicans DC. by HPTLC
Table 1
Method validation parameters for the estimation of psoralen and heraclenol by HPTLC densitometry.
| Parameter | Results | Psoralen (1) | Heraclenol (2) |
| Accuracy (%) | 85.64 | 93.51 | Precision (% RSD) | | | CV (instrument precision) | 0.037948 | | CV (method precision) | 1.31 | 1.14 | Interday precision | 0.73 | 0.80 | Intraday precision | 0.68 | 0.75 | Limit of detection | 0.86 µg | 2.79 µg | Limit of quantification | 2.62 µg | 4.82 µg | Specificity | Specific | Specific | Correlation coefficient | 0.973 | 0.964 | Linearity range |
1–5 µg spot−1 | 1–4 µg spot−1 | Linear regression coefficient | | | Slope | | | -intercept | | | value | 0.0134 | 0.0179 |
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